Application of SNRPB as biomarker in prognosis evaluation of hepatocellular carcinoma and kit containing biomarker
A biomarker and hepatocellular carcinoma technology, applied in the field of molecular markers of hepatocellular carcinoma, to reduce the possibility of recurrence and metastasis, improve the quality of life, and prolong the survival time
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Embodiment 1
[0047] Embodiment 1: TCGA database analysis
[0048] Using the TCGA visualization database GEPIA (Gene Expression Profiling Interactive Analysis) to analyze the expression level of SNRPB in 369 cases of hepatocellular carcinoma samples and 50 cases of normal samples, the results are as follows figure 1 shown.
[0049] Depend on figure 1 It can be seen that the expression of SNRPB in hepatocellular carcinoma tissues is higher than that in normal tissues.
[0050] Kaplan-Meier survival analysis was used to analyze the relationship between SNRPB expression and prognosis in TCGA database hepatocellular carcinoma samples. According to the median of SNRPB expression value, the samples were divided into two groups with high and low expression. The result is as figure 2 shown.
[0051] Depend on figure 2 It can be seen that the overall survival rate of patients with high expression of SNRPB is significantly reduced.
Embodiment 2
[0052] Example 2: Detection of SNRPB expression in hepatocellular carcinoma and paracancerous samples by fluorescent quantitative PCR
[0053] 2.1 Organization source
[0054] Sixty-six hepatocellular carcinoma tissues and 21 paired paracancerous tissues were obtained from the Department of Hepatobiliary Surgery of the Affiliated Hospital of Guilin Medical College. The operation time was from December 2010 to December 2012, and the follow-up period ended in April 2016.
[0055] 2.2 RNA extraction
[0056] (1) Hepatocellular carcinoma tissues and paracancerous tissues frozen in liquid nitrogen were taken and ground into powder in a pre-cooled mortar, then 1 mL of lysis solution Trizol was added, and the lysate was obtained by standing at room temperature for 5 minutes.
[0057] (2) Transfer the lysate into a 1.5mL centrifuge tube, add 0.2mL chloroform, shake the centrifuge tube vigorously, let it stand at room temperature for 10min, then centrifuge at 12000rpm for 15min, take...
Embodiment 3
[0080] Example 3: Clinical Significance of High Expression of SNRPB
[0081] Kaplan-Meier survival analysis was performed using GraphPad Prism 7.0 software. According to the median of SNRPB expression values, the samples were divided into two groups with high expression and low expression. result Figure 4 shown.
[0082] Depend on Figure 4 It can be seen that when the SNRPB expression data in the test result is above the median expression value, it is SNRPB high expression; in the test result, the SNRPB expression data is below the expression value median, then it is SNRPB low expression. The high expression of SNRPB is significantly related to the decrease of the overall survival rate in patients with hepatocellular carcinoma, and the prognosis is high mortality rate; postoperative recurrence and metastasis rate are high, and it is recommended that patients undergo active follow-up examination and treatment. The low expression of SNRPB is significantly correlated with t...
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