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Heptapeptide with function of resisting Abeta protein aggregation and application of heptapeptide as well as gene for encoding polypeptide

A technology of β protein and heptapeptide, which is applied in the field of heptapeptide and its application and the gene encoding the synthetic polypeptide, can solve the problems of lack of specificity and achieve the effects of improving memory, major social and economic benefits, and improving medical conditions

Active Publication Date: 2018-10-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the treatment of AD has always lacked effective drugs that are highly specific, relieve and reverse the disease, and improve the prognosis of the disease.

Method used

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  • Heptapeptide with function of resisting Abeta protein aggregation and application of heptapeptide as well as gene for encoding polypeptide
  • Heptapeptide with function of resisting Abeta protein aggregation and application of heptapeptide as well as gene for encoding polypeptide
  • Heptapeptide with function of resisting Abeta protein aggregation and application of heptapeptide as well as gene for encoding polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Synthesis of Peptide WW-7 by Solid Phase Synthesis of Peptide

[0042] 1. Resin selection

[0043] (1) Using the standard Fomc protocol, starting with 0.0125mmol 2-chlorotrityl chloride resin resin (Tianjin Nankai Synthetic Technology Co., Ltd.), according to the amino acid sequence Trp-Asp-Gln-Trp-Cys-Ile-Trp from the C-terminal to the N According to the sequence characteristics of the terminal, 0.3mol of the first Fmoc protected amino acid was added, DCC and 5% (mass fraction) DMAP were added to the reactor to shake the reaction, and the resin was washed with methylpyrrolidone (NMP) to remove excess protected amino acids.

[0044] (2) Using the standard Fomc scheme, initially select 0.0125mmol Wang resin, according to the sequence characteristics of the amino acid sequence Trp-Asp-Gln-Trp-Cys-Ile-Trp from the C-terminal to the N-terminal, add 0.3mol of the first Fmoc protected amino acid , DCC and 5% (mass fraction) DMAP are added to the reactor for shaking reaction,...

Embodiment 2

[0052] Anti-Aβ42 Protein Aggregation Activity Experiment of Polypeptide WW-7 in Vitro

[0053] 1. Experimental method

[0054] Preparation of culture medium: High glucose medium (DMEM), fetal bovine serum (FBS), L-glutamine were prepared according to the mass ratio of 8.75:1:0.25 respectively; 1wt% double antibodies (penicillin and streptomycin) were added at the same time , 0.1 wt% Hygromycin B and 0.05 wt% Blasticidin S antibiotics.

[0055] Preparation of 0.05mM and 0.5mM polypeptide (WW-7) solutions: Weigh 10.4mg of polypeptide WW-7, dissolve it with 10mL of medium, pass through a 0.22μm filter head, the concentration of the mother solution is 1mM, and then use the medium to dissolve The mother liquor was diluted to the concentration required for the experiment.

[0056] Preparation of 1 mg / mL tetracycline solution: Weigh 20 mg of tetracycline, prepare with 10 mL of PBS buffer, pass through a 0.22 μm filter head, and store at -20°C in the dark for future use.

[0057] F...

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Abstract

The invention discloses a heptapeptide with a function of resisting Abeta42 protein aggregation and application of the heptapeptide as well as a gene for encoding the polypeptide. The synthetic peptide has the efficacy of obviously resisting the Abeta42 protein aggregation, and further has the effects of improving memory and delaying disease progress of Alzheimer's disease; the synthetic peptide can be widely applied to preparation of a medicine or food for resisting the Abeta42 protein aggregation, or is applied to preparation of a medicine or food for preventing or treating the Alzheimer's disease, and can further effectively prevent and treat neurodegenerative diseases including AD disease so as to improve medical conditions of the neurodegenerative diseases; the heptapeptide has greatsocial and economic benefits.

Description

technical field [0001] The invention relates to the technical field of polypeptides, in particular to a heptapeptide against Aβ protein aggregation and its application and a gene encoding the synthetic polypeptide. Background technique [0002] Alzheimer's disease (AD), also known as senile dementia, is a central nervous degenerative disease induced by multiple factors. The clinical manifestations of AD are gradual loss of memory, inability to take care of oneself in daily life, incontinence of urine and feces in the late course of the disease, symptoms of mutism, limb stiffness, primitive reflexes such as strong grasping, groping and sucking, and eventually coma and death due to infection and other reasons. The course of AD is about ten years, showing a completely helpless living state from onset to death. The chronic nature of AD, and its assault on the fragile structures that shelter self-knowledge, imposes an enormous emotional and financial burden on the patient, the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12N15/11A61K38/08A61P25/28A23L33/18
CPCA23V2002/00A61K38/00A23L33/18A61P25/28C07K7/06A23V2200/322A23V2250/55
Inventor 任娇艳杨柳
Owner SOUTH CHINA UNIV OF TECH
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