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Identifying method of Acipenser baerii and Acipenser baerii hybrid

An identification method and sturgeon technology, applied in the field of molecular biology, can solve problems such as complex sample preparation

Active Publication Date: 2018-10-16
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Using this method for identification, although the operation is simple and easy to learn and master, but the sample preparation is complicated, the band difference between samples is small, and it is easily affected by the sample collection method and various conditions in the experimental process. Therefore, in recent years, There are few reports on the identification of sturgeons, and they are mostly used for the assessment and determination of the individual health of sturgeons under artificial feeding conditions.

Method used

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  • Identifying method of Acipenser baerii and Acipenser baerii hybrid
  • Identifying method of Acipenser baerii and Acipenser baerii hybrid
  • Identifying method of Acipenser baerii and Acipenser baerii hybrid

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Embodiment

[0067] The identification method of Siberian sturgeon and western miscellaneous comprises the following steps:

[0068] (1) Use the sturgeon DNA to be identified as a template (the extraction method adopts the extraction method of total genomic DNA in the previous 2), and use the Siberian sturgeon primer pair as primers for PCR amplification. The reaction system for PCR amplification is 1 μL of DNA template, Mix 12.5 μL, ddH 2 O 10.5 μL, upstream primer 0.5 μL, downstream primer 0.5 μL. The PCR reaction conditions were as follows: pre-denaturation at 95°C for 5 min; then 35 cycles, each cycle including denaturation at 94°C for 40 s, annealing at 50°C for 35 s, and extension at 72°C for 50 s; finally, extension at 72°C for 10 min. After gel electrophoresis of the PCR product with 120v 1.5% agarose, the amplified fragment was detected with ultraviolet light. If there is a 215bp amplification product, it is judged as Siberian sturgeon or Western hybrid;

[0069] Or use the Stu...

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Abstract

The invention discloses an identifying method of Acipenser baerii and Acipenser baerii hybrid. The identifying method comprises the following steps: (1) taking to-be-identified sturgeon DNA as a template: a, taking an Acipenser baerii primer pair as a primer to perform PCR amplification, and judging as Acipenser baerii or Acipenser baerii hybrid if a 215bp amplification product appears; or b, taking a Acipenser schrenckii primer pair as a primer to perform PCR amplification, and judging as Acipenser schrenckii or Acipenser schrenckii hybrid if a 252bp amplification product appears; (2) furtheridentifying sturgeon DNA which is judged as Acipenser baerii or Acipenser baerii hybrid: taking to-be-identified sturgeon DNA as a template, taking a HLJSX48 primer pair as a primer to perform PCR amplification, judging as Acipenser baerii hybrid if a 163 bp amplification product appears, otherwise, judging as Acipenser baerii. The identifying method can be used for simply and quickly identifyingafter taking Acipenser baerii as a female parent and taking Acipenser schrenckii as a male parent to perform hybridization on generation.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to an identification method for Siberian sturgeon and western miscellaneous species. Background technique [0002] Sturgeon's sturgeon and Siberian sturgeon are two important cultured species in my country. Under natural conditions, Sturgeon's sturgeon is only distributed in the Heilongjiang (Amur River) water system. It is a unique species and an important economic fish in Heilongjiang. Its growth rate is fast, but its disease resistance is poor, it is not resistant to transportation, and it is too dependent on live bait It is difficult to domesticate and has a high mortality rate during the breeding process, which increases the risk of the sturgeon farming industry. The Siberian sturgeon is mainly distributed in Central Asia and Eastern Europe, and a small amount is distributed in the Irtysh River system in my country. Its growth rate is slow, but it has strong diseas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888
Inventor 杨世勇赵仲孟刘钊黄小丽苗懿杜宗君罗伟陈虎陈德芳冯杨段靖熊关庆任妍李涛张谨啸张瀚艺朱凌威王云维陈雨薇杨磊谭淦刘洪李卢永瑞
Owner SICHUAN AGRI UNIV
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