Acipenser schrenckii sperm cryopreservation solution and preservation method
A technology of cryopreservation solution and preservation method, which is applied in the field of stylized sturgeon breeding to achieve the effects of improving efficiency, improving sperm motility, and increasing cleavage rate
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[0028] A kind of cryopreservation liquid of sturgeon sperm, this cryopreservation liquid is made by comprising the following components by weight: 1.8 parts of sodium chloride, 2.6 parts of potassium chloride, 8.2 parts of ethylene glycol, 3.5 parts of sodium dihydrogen phosphate, 0.54 parts of pyridoxine, 0.26 parts of GSH, 0.05 parts of HA, 0.13 parts of SDS, 0.74 parts of Vc.
[0029] The preparation method of the sperm cryopreservation solution of Stryn's sturgeon comprises the following steps: dissolving each component in distilled water of 5 times the amount, and mixing evenly.
[0030] A method for preserving the sperm of Acipenser's sturgeon, comprising the following steps: lowering the temperature of the cryopreservation solution to 5-6°C, then mixing and shaking the semen of Acipenser's sturgeon with 5 times the amount of the cryopreservation solution, Then divide the mixed solution, pre-cool the small-packed mixed solution at 1-3°C for 30 minutes, then place the pre...
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