Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preservation method and recovery method of Acipenser schrencki sperms

A preservation method, the technology of Sturgeon's sturgeon, which is applied to the preservation of human or animal bodies, germ cells, vertebrate cells, etc., to achieve the effects of protecting sperm, smooth recovery process, and ensuring structural integrity

Inactive Publication Date: 2021-05-18
SOUTHWEST UNIVERSITY
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sperm preservation and sperm quality of Sturgeon's sturgeon still need to be further studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preservation method and recovery method of Acipenser schrencki sperms
  • Preservation method and recovery method of Acipenser schrencki sperms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for preserving spermatozoa of Stren's sturgeon, comprising the steps of:

[0024] (1) Mix the Sturgeon's sperm with 8 times the amount of antifreeze, and then pack them into sterile containers;

[0025] (2) Pre-cool the mixed solution of Stryn's sturgeon sperm after subpackaging in step (1) to 5°C, store it at a constant temperature for 15 minutes, then cool the mixed solution to -20°C at a cooling rate of -1°C / min, Store at constant temperature for 10 minutes, then cool the mixture to -50°C at a cooling rate of -3°C / min, store at a constant temperature for 5 minutes, then cool the mixture to -100°C at a cooling rate of -5°C / min, and store at a constant temperature 3 minutes;

[0026] (3) Put the mixed solution obtained in step (2) into liquid nitrogen, and it is ready.

[0027] The antifreeze in the described step (1) includes 1.8 parts of sodium chloride, 1.5 parts of potassium chloride, 6.5 parts of sodium citrate, 1.2 parts of sodium hydrogen phosphate, 0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preservation method and recovery method of Acipenser schrencki sperms. The preservation method comprises the following steps: (1) uniformly mixing Acipenser schrencki sperms with 5-10 times of an antifreezing agent, and then subpackaging the mixture into sterile containers; (2) pre-cooling the mixed liquid of the sperms of the Acipenser schrencki subpackaged in the step (1) to 3-5 DEG C, conducting preserving at a constant temperature for 10-20 minutes, cooling the mixed liquid to -15 DEG C to -20 DEG C at a cooling speed of -1 DEG C / min, conducting preserving at a constant temperature for 5-10 minutes, cooling the mixed liquid to -40 DEG C to -50 DEG C at a cooling speed of -3 DEG C / min, conducting preserving at a constant temperature for 1-5 minutes, cooling the mixed solution to -80 DEG C to -100 DEG C at a cooling speed of -5 DEG C / min, and conducting preserving for 1-5 minutes at a constant temperature; and (3) putting the mixed solution obtained in the step (2) into liquid nitrogen to obtain a finished product. According to the preservation method for the sperms of the acipenser schrencki, the sperms are subjected to cryopreservation in a subpackaging and cooling constant-temperature mode, so that the generation of crystals of the sperms in a crystallization dangerous zone of 0-40 DEG C is reduced, and the structural integrity of cells is ensured.

Description

technical field [0001] The invention belongs to the field of sturgeon breeding, and in particular relates to a preservation method and a recovery method of sturgeon sperm. Background technique [0002] Sturgeon's sturgeon belongs to Osteophytes, Atenopterygia, Sclerophyta, and Sturgeoniformes. It is mainly distributed in the Amur River (Heilongjiang, China) basin. Because of its advantages such as fast growth, strong disease resistance, and high economic value, it has been It has become a popular cultured species in China, accounting for 50% of China's total sturgeon production, and there is still an increasing trend. However, due to extensive breeding techniques and high breeding density, the frequency of disease outbreaks has increased, which has greatly hindered the healthy and sustainable development of sturgeon aquaculture. In recent years, scientific researchers have done a lot of work in the protection of Acipenser schneidi resources, artificial breeding, artificial ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N1/02C12N5/076
CPCA01N1/0221C12N5/061
Inventor 朱成科吕光俊蒋礼杨成年阳龙江唐征县韩璐璐彭小倩
Owner SOUTHWEST UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com