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Digital multiple-nucleic-acid detection method on basis of encoding micro-sphere reactors

A technology of encoding microspheres and detection methods, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of multiple PCR mutual interference, reduce non-specific hybridization reactions, and avoid insufficient substrates.

Inactive Publication Date: 2018-10-16
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

[0007] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a digital multiple nucleic acid detection method based on a coded microsphere reactor, to improve the mutual interference of traditional multiple PCR, and to improve the single detection weight of digital PCR. Number, to achieve multiple digital nucleic acid quantitative detection

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[0027] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0028] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0029] The present invention is further illustrated below by specific examples. However, the specific details of the embodiments are only used to explain the present invention, and should not be construed as limiting the general technical solution of the present invention.

[0030] This embodiment provides a digital multiple nucleic acid detection method based on a coded microsphere reactor, comprising the following steps:

[0031] 1) Sample nucleic acid extraction: Obtain microorganisms from various sources as samples, disperse them in the water phase, and make a suspension. Afte...

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Abstract

The invention discloses a digital multiple-nucleic-acid detection method on the basis of encoding micro-sphere reactors. The digital multiple-nucleic-acid detection method includes steps of extractingsample nucleic acid; designing primers; labeling the primers; carrying out hybrid capture; generating micro-drop; carrying out amplification reaction; carrying out scanning detection and the like. The digital multiple-nucleic-acid detection method has the advantages that multiple-gene detection merits of hybridization technologies and high-sensitivity quantitative detection merits of digital PCR(polymerase chain reaction) are combined with one another, accordingly, gap in the technical field of multiple-gene quantitative detection can be filled, and the digital multiple-nucleic-acid detection method has profound influence.

Description

technical field [0001] The invention relates to the field of molecular detection, in particular to a digital multiplex nucleic acid detection method based on a coded microsphere reactor. Background technique [0002] The principle of multiplex polymerase chain reaction (Polymerase Chain Reaction, PCR) is to add multiple pairs of specific primers in the reaction system at the same time, and simultaneously amplify the DNA fragments of multiple entries. Simultaneous detection of multiple microorganisms can be achieved based on the fragment size of the product or by molecular hybridization. Since the concept of multiplex PCR was first proposed in 1988, after years of development, multiplex PCR technology has gradually matured. Currently, multiplex PCR detection methods mainly include capillary electrophoresis (or gel electrophoresis), liquid phase chip, and mass spectrometry. Capillary electrophoresis is to separate and detect PCR products according to their different amplified...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2537/143C12Q2563/159C12Q2531/113
Inventor 白鹏利王策
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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