Method for improving bifidobacterium cell density and metabolic products via high-density mixed fermentation
A bifidobacterium cell, high-density fermentation technology, applied in the field of microbiology, can solve the problems of bifidobacteria product development, production, storage and transportation difficulties, acid resistance, heat resistance, poor curdling performance, high cost, etc., to achieve the improvement of cell Density and metabolites, low price, growth-promoting effect
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Embodiment 1
[0057] A method for increasing bifidobacteria cell density and metabolites through mixed high-density fermentation, comprising the following steps:
[0058] 1. For the activation of strains, take the Bifidobacterium adolescent strain 23001 preserved in our laboratory and inoculate it on the Bifidobacterium slant medium. Incubate at 37°C for 24 hours and set aside. Take the Bacillus natto strain ZJFD4 preserved in our laboratory and inoculate it on the slant medium of aerobic bacteria (Bacillus natto). Incubate at 37°C for 24 hours and set aside.
[0059] 2. Preparation of seed liquid, transfer bifidobacteria and aerobic bacteria (Bacillus natto) activated on the slant to Bifidobacterium seed culture medium and aerobic bacteria (Bacillus natto) seed culture medium respectively, and carry out seed preparation . Bifidobacterium seed culture conditions are: static anaerobic culture, 37 ℃, culture 24h to logarithmic growth phase, adjust the number of viable bacteria in the seed ...
Embodiment 2
[0063] A method for increasing bifidobacteria cell density and metabolites through mixed high-density fermentation, comprising the following steps:
[0064] 1. For the activation of strains, take the Bifidobacterium adolescent strain 23001 preserved in our laboratory and inoculate it on the Bifidobacterium slant medium. Incubate at 37°C for 24 hours and set aside. Take the Bacillus subtilis strain 10012 preserved in our laboratory and inoculate it on the slant medium of aerobic bacteria (Bacillus subtilis). Incubate at 37°C for 24 hours and set aside.
[0065] 2. Seed liquid preparation, the bifidobacteria and Bacillus subtilis activated on the slant were transferred to the bifidobacterium seed culture medium and the aerobic bacteria (Bacillus subtilis) seed culture medium respectively, and the seeds were prepared. Bifidobacterium seed culture conditions are: static anaerobic culture, 37 ℃, culture 24h to logarithmic growth phase, adjust the number of viable bacteria in the ...
Embodiment 3
[0069] A method for increasing bifidobacteria cell density and metabolites through mixed high-density fermentation, comprising the following steps:
[0070] 1. For the activation of strains, take the Bifidobacterium adolescent strain 23001 preserved in our laboratory and inoculate it on the Bifidobacterium slant medium. Incubate at 37°C for 24 hours and set aside. Take the Bacillus licheniformis strain 10093 preserved in our laboratory and inoculate it on the slant medium of aerobic bacteria (Bacillus licheniformis). Incubate at 37°C for 24 hours and set aside.
[0071] 2. Seed liquid preparation, the bifidobacteria and bacillus licheniformis activated on the slope were transferred to the bifidobacterium seed culture medium and the aerobic bacteria (Bacillus licheniformis) seed culture medium respectively, and the seeds were prepared. Bifidobacterium seed culture conditions are: static anaerobic culture, 37 ℃, culture 24h to logarithmic growth phase, adjust the number of via...
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