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Method for improving bifidobacterium cell density and metabolic products via high-density mixed fermentation

A bifidobacterium cell, high-density fermentation technology, applied in the field of microbiology, can solve the problems of bifidobacteria product development, production, storage and transportation difficulties, acid resistance, heat resistance, poor curdling performance, high cost, etc., to achieve the improvement of cell Density and metabolites, low price, growth-promoting effect

Inactive Publication Date: 2018-10-12
GUIZHOU INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since bifidobacteria are obligate anaerobic bacteria with poor acid resistance, heat resistance, and milk curdling properties, special anaerobic culture equipment is required for separate cultivation, which is troublesome to operate and high in cost, and the cell density of frequent culture cannot reach a high level. This has brought great difficulties to the development, production, storage and transportation of bifidobacteria products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A method for increasing bifidobacteria cell density and metabolites through mixed high-density fermentation, comprising the following steps:

[0058] 1. For the activation of strains, take the Bifidobacterium adolescent strain 23001 preserved in our laboratory and inoculate it on the Bifidobacterium slant medium. Incubate at 37°C for 24 hours and set aside. Take the Bacillus natto strain ZJFD4 preserved in our laboratory and inoculate it on the slant medium of aerobic bacteria (Bacillus natto). Incubate at 37°C for 24 hours and set aside.

[0059] 2. Preparation of seed liquid, transfer bifidobacteria and aerobic bacteria (Bacillus natto) activated on the slant to Bifidobacterium seed culture medium and aerobic bacteria (Bacillus natto) seed culture medium respectively, and carry out seed preparation . Bifidobacterium seed culture conditions are: static anaerobic culture, 37 ℃, culture 24h to logarithmic growth phase, adjust the number of viable bacteria in the seed ...

Embodiment 2

[0063] A method for increasing bifidobacteria cell density and metabolites through mixed high-density fermentation, comprising the following steps:

[0064] 1. For the activation of strains, take the Bifidobacterium adolescent strain 23001 preserved in our laboratory and inoculate it on the Bifidobacterium slant medium. Incubate at 37°C for 24 hours and set aside. Take the Bacillus subtilis strain 10012 preserved in our laboratory and inoculate it on the slant medium of aerobic bacteria (Bacillus subtilis). Incubate at 37°C for 24 hours and set aside.

[0065] 2. Seed liquid preparation, the bifidobacteria and Bacillus subtilis activated on the slant were transferred to the bifidobacterium seed culture medium and the aerobic bacteria (Bacillus subtilis) seed culture medium respectively, and the seeds were prepared. Bifidobacterium seed culture conditions are: static anaerobic culture, 37 ℃, culture 24h to logarithmic growth phase, adjust the number of viable bacteria in the ...

Embodiment 3

[0069] A method for increasing bifidobacteria cell density and metabolites through mixed high-density fermentation, comprising the following steps:

[0070] 1. For the activation of strains, take the Bifidobacterium adolescent strain 23001 preserved in our laboratory and inoculate it on the Bifidobacterium slant medium. Incubate at 37°C for 24 hours and set aside. Take the Bacillus licheniformis strain 10093 preserved in our laboratory and inoculate it on the slant medium of aerobic bacteria (Bacillus licheniformis). Incubate at 37°C for 24 hours and set aside.

[0071] 2. Seed liquid preparation, the bifidobacteria and bacillus licheniformis activated on the slope were transferred to the bifidobacterium seed culture medium and the aerobic bacteria (Bacillus licheniformis) seed culture medium respectively, and the seeds were prepared. Bifidobacterium seed culture conditions are: static anaerobic culture, 37 ℃, culture 24h to logarithmic growth phase, adjust the number of via...

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Abstract

The invention discloses a method for improving bifidobacterium cell density and metabolic products via high-density mixed fermentation. A good anaerobic environment can be provided for bifidobacteriasince aerobic bacteria can consume oxygen in a culture solution in mixed fermentation of the bifidobacteria and the aerobic bacteria. Under a same inoculum size and same culture conditions, taking bifidobacteria and bacillus natto as examples, viable count of the bifidobacteria in fermentation liquid in a control group is 4.9*10<9>cuf / mL, while viable count of the bifidobacteria in the high-density mixed fermentation method provided by the invention is 6.7*10<9>cuf / mL, 36.7% greater than that of the control group. Based upon pH value of the fermentation liquid, metabolic situations of bacteriacan be reflected, and more metabolic products are generated in the fermentation liquid as the pH value is lower. In the control group, the pH value is 5.91, while the pH value of the fermentation liquid is 5.22 in the high-density mixed fermentation method provided by the invention, 13.0% lower than that of the control group, showing that the bacteria, obtained from mixed culture, are better thana bifidobacterium single-culture method in capacity of secreting the metabolic products.

Description

technical field [0001] The invention belongs to the field of microbiology, and in particular relates to a method for increasing bifidobacteria cell density and metabolites through mixed high-density fermentation. Background technique [0002] Bifidobacterium was first isolated from the feces of breast-fed infants by Dr. Tissier of the Pasteur Institute in France in 1899. It is one of the most important physiological bacteria in the intestines of humans and animals. Bifidobacteria can regulate and maintain the micro-ecological balance of the human body, synthesize a variety of vitamins and other nutrients necessary for the human body, antagonize a variety of intestinal pathogenic microorganisms, have anti-infection, two-way regulation of intestinal function, enhance human immunity, improve nutrition, etc. It has a variety of physiological functions and is one of the important probiotics. [0003] Bifidobacteria are Gram-positive, strictly anaerobic and polymorphic bacteria w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/10C12R1/125C12R1/07C12R1/01
CPCC12N1/20
Inventor 刘晓柱李银凤黄名正刘晓辉于志海
Owner GUIZHOU INST OF TECH
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