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Detecting method of traditional Chinese medicine composition for treating coronary heart disease

A detection method and technology of coronary heart disease, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of incurability, high price, high toxicity and side effects, etc.

Inactive Publication Date: 2018-10-02
INNER MONGOLIA TIANQI HAN&MONGOLIA PHARMA CO +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, Western medicine is commonly used in the treatment of coronary heart disease, which is expensive and has very high toxicity and side effects. However, the current application of traditional Chinese medicine treatment or adjuvant treatment can only alleviate the suffering of patients, but cannot cure it, and the curative effect is not satisfactory.

Method used

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  • Detecting method of traditional Chinese medicine composition for treating coronary heart disease
  • Detecting method of traditional Chinese medicine composition for treating coronary heart disease
  • Detecting method of traditional Chinese medicine composition for treating coronary heart disease

Examples

Experimental program
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Effect test

Embodiment 3

[0115] The content determination of embodiment three batches of test samples

[0116] 1. Determination of the test product

[0117] Dima C 18 Column (5μm, 4.6mm×250mm); with octadecylsilane bonded silica gel as filler; with 0.017% formic acid solution as mobile phase A, with methanol-acetonitrile (30:10) as mobile phase B, press The gradient elution is specified in the table; the flow rate is 1ml per minute; the detection wavelength is 286nm; the column temperature is 30°C. The number of theoretical plates should not be less than 4000 based on the peak of salvianolic acid B. See the table below for the elution method.

[0118] gradient elution table

[0119]

[0120] Preparation of reference substance solution: Accurately weigh an appropriate amount of salvianolic acid B reference substance, put it in a measuring bottle, add 75% methanol to make a solution containing 60 μg of salvianolic acid B per 1 ml, as the reference substance solution.

[0121] The preparation of ...

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Abstract

The invention discloses a detecting method of a traditional Chinese medicine composition for treating coronary heart disease. A formula is prepared from radix salviae miltiorrhizae, fructus piperis longi, lignum aquilariae resinatum, lignum dalbergiae odoriferae, semen myristicae, rhizoma kaempferiae, fructus choerospondiatis, lignum santali albi, fructus hippophae and lignum pterocarri accordingto a certain weight proportion and is prepared into capsules; the fructus piperis longi and the lignum aquilariae resinatum are distinguished by using TLC (Thin-Layer Chromatography); the content of salvianolic acid B by using HPLC (High Performance Liquid Chromatography). The detecting method disclosed by the invention is capable of effectively ensuring the product quality during production and using of the traditional Chinese medicine composition, so that the curative effect of medicine products is ensured.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a detection method of a traditional Chinese medicine composition for treating coronary heart disease, and belongs to the technical field of medicine. Background technique [0002] The incidence of coronary heart disease in middle-aged and elderly people is getting higher and higher, and it shows a trend of gradually becoming younger. Patients with coronary heart disease are usually accompanied by palpitations, angina pectoris, cardiac insufficiency, etc., unable to engage in physical labor, seriously unable to take care of themselves, and even die. At present, Western medicines are commonly used in the treatment of coronary heart disease, which are expensive and have very large toxic and side effects. However, the current application of traditional Chinese medicine treatment or adjuvant treatment can only alleviate the suffering of patients, but cannot cure them, and the curative effect...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02
CPCG01N30/02G01N30/90
Inventor 韩风雨佘宜寰李洪泽于秀玲齐平
Owner INNER MONGOLIA TIANQI HAN&MONGOLIA PHARMA CO
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