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Application of TCC to prevention of colony diffuse growth in colony count determination

A technology for the determination of the total number of colonies and colonies, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve problems such as poor results.

Inactive Publication Date: 2018-10-02
德清县食品药品检验所
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AI Technical Summary

Problems solved by technology

However, this method is not effective in preventing the diffuse growth of colonies

Method used

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  • Application of TCC to prevention of colony diffuse growth in colony count determination
  • Application of TCC to prevention of colony diffuse growth in colony count determination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 TTC inhibits the diffuse growth of bacterial colonies

[0012] 1 Materials and methods

[0013] 1.1 Materials

[0014] 1.1.1 Plate count agar medium was provided by Guangdong Huankai Microbial Technology Co., Ltd.

[0015] 1.1.2 Analytical grade 2,3,5-triphenyltetrazolium chloride (TTC), provided by Beijing Solarbio Technology Co., Ltd.

[0016] 1.1.3 Bacillus cereus (CMCC(B)63303), Escherichia coli (ATCC25922), Staphylococcus aureus (CMCC(B)26003) were provided by Guangdong Huankai Microbial Technology Co., Ltd.;

[0017] 1.2 Method

[0018] 1.2.1 Preparation of 1% TTC Weigh 1g of TTC, add 99mL of distilled water, dissolve completely, sterilize with a 0.22μm filter membrane, and store in a refrigerator at 4°C in the dark.

[0019] 1.2.2 Bacterial solution preparation Bacillus cereus, Escherichia coli and Staphylococcus aureus were inoculated on nutrient agar slant medium respectively, and cultured at 36±1°C for 24 hours. Take the above-mentioned slant la...

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Abstract

The invention provides application of TCC to prevention of colony diffuse growth in colony count determination. The method includes: taking Bacillus cereus, Escherichia coli and Staphylococcus aureusof appropriate dilution, adding 0.01%, 0.05%, 0.08%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.8%, and 1.0% TTC aqueous solution plate count agar, at the same time taking count agar without TTC as control, andobserving the growth condition of bacteria. In colony count determination, if a sample possibly contains Bacillus colonies growing diffusely on an agar medium surface, TTC with a concentration of 0.2%-0.3% can be added to the agar to prevent incalculability situation caused by diffusion of colonies.

Description

technical field [0001] The invention belongs to the field of measuring the total number of bacterial colonies, in particular to a method for preventing the diffuse growth of bacillus in the determination of the total number of bacterial colonies. Background technique [0002] As the basic inspection item of food microbiology inspection, the total number of bacterial colonies is of great significance. It is used to determine the degree of food contamination by bacteria and the hygienic quality, and to reflect whether the food meets the hygienic requirements during the production process, so as to make appropriate decisions on the inspected samples. health evaluation. Nowadays, there are many types of food, a wide variety of raw materials, and complex processing techniques. Especially high-starch foods such as cakes and pastries are highly likely to be contaminated by Bacillus, and it is difficult to completely sterilize them during processing. There will be a phenomenon of d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 郑连宝丁琦陈文祥嵇林凤施华标
Owner 德清县食品药品检验所
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