Preparation method for extract of total flavonoids in kernel of Prinsepia utilis

Abstract

The invention discloses a preparation method for the extract of total flavonoids in the kernel of Prinsepia utilis. The preparation method comprises the following steps: degreasing: removing grease indried and crushed kernels of Prinsepia utilis by using a degreasing agent, and evaporating the degreasing agent so as to obtain a Prinsepia utilis kernel material; reflux extraction: subjecting the Prinsepia utilis kernel material to reflux extraction by using an aqueous methanol solution with a volume concentration of 40 to 95 vol% or an aqueous methanol solution n with a volume concentration of40 to 95 vol% so as to obtain liquid extract, and drying the liquid extract by evaporation so as to obtain crude extract; and separation and purification: separating and purifying the crude extract by using a macroporous resin column and carrying out enrichment so as to obtain the extract containing the total flavonoids. The preparation method has the technical effects that the content of the total flavonoids in the extract is allowed to reach 30 wt% or more; and time for separation and purification is substantially shortened in the process of preparation.

Description

technical field [0001] The invention relates to a preparation method of total flavonoids extract of green thorn nuts in the technical field of extraction. Background technique [0002] Flavonoids (Flavonoids Compounds) are plant secondary metabolites, which widely exist in natural plants in the form of free or sugar-bound glycosides. Flavonoids exhibit a variety of pharmacological activities, can prevent and treat diseases of the cardiovascular and cerebrovascular systems and respiratory systems, have anti-inflammatory, antibacterial, hypoglycemic, anti-oxidation, anti-radiation, anti-cancer, anti-tumor and enhance immunity, etc. pharmacological effects. In recent years, the study of flavonoids has entered a new level. With the in-depth research on the relationship between their structures and functions, some mechanisms of action of flavonoids have been discovered, which provides a basis for their application in the fields of skin care products, medicine, and food. The the...

AI Technical Summary

Problems solved by technology

[0006] At present, the extraction of the total flavonoids in the green thorn nuts is directly extracted with ethanol or methanol to obtain a crude extract, and then separated and purified to obtain the green thorn nut extract. There is no degreasing process for the preparation of high total flavonoids According to the research report of the green thorn nut extract, the total flavonoid content in the green thorn nut extract is low, ranging from 0.04 to 8wt.%, and the green thorn nut contains very high oil, generally accounting for 30wt. .%, which brings the following technical problems: the first, the oil will reduce the adsorption efficiency of the macroporous resin during purification, prolong the column chromatography time, and the macroporous resin column cannot be reused due to the adsorption of oil; the second, There is a relatively high content of oil in the resulting product, which is not conducive to dissolution and limits its application

[0011] According to the traditional process method, the crude extract obtained by extracting the green thorn nuts without degreasing treatment is very oily, and it will cause serious blockage when passing through the D101 macroporous resin column. The separation and purification time exceeds 36h, and the macroporous Resin recycling brings inconvenience

[0012] If no degreasing measures are taken and the extraction is carried out directly, not only the yield of the extract is low, but also the separation and purification will be time-consuming and laborious due to the high oil content, and the total flavonoid content of the flavonoid effective fraction in the finally enriched extract is low

Compared with other research workers' researches on the extraction of green thorn nuts, there is no report that the total flavonoid content exceeds 30wt.%.

Images