Microbial sample preservation solution for genetic detection and its preparation method, kit and application

Abstract

The invention provides a microbial sample preservation solution for gene detection, a preparation method, a kit and an application thereof, and belongs to the technical field of bioengineering. The microbial sample preservation solution for gene detection provided by the present invention has more effective and comprehensive microbial inactivation ability; the use of lower concentration of ethanol can more thoroughly inactivate bacteria during sample storage, and avoid bacteria caused by high concentration of ethanol Inactivation fails due to stress response. On the premise of not destroying the cell structure, it has good permeability, effectively destroys the protein activity and various nuclease activities of bacteria and viruses, so that it no longer has the ability to infect or proliferate, and the inactivation effect is thorough. Using a glycerin-containing cell protectant and a physiological concentration buffer can maintain the cell structure of microorganisms and avoid cell structure rupture, thereby protecting the integrity of nucleic acids in cells; there are no highly toxic components or volatile components, and no volatilization will occur during use Sexual Toxic.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a microbial sample preservation solution for gene detection, a preparation method, a kit and an application thereof. Background technique [0002] At present, the oral / fecal bacterial preservation solutions on the market are mainly composed of ethanol / methanol / SDS, and are prepared into weakly alkaline isotonic solutions, which can kill the bacteria in the sample, but will cause damage to the cell structure, resulting in Cell contents are released and cannot be detected by immunological or other methods. At the same time, methanol\guanidine isothiocyanate in the preservation solution is volatile or highly toxic, and it is easy to cause volatilization during use and cause harm to operators. [0003] The existing preservation solution is inactivated with high-purity ethanol; some bacteria will have a stress response to high-concentration ethanol, resulting in a reduced inac...

AI Technical Summary

Problems solved by technology

[0002] At present, the oral / fecal bacterial preservation solutions on the market are mainly composed of ethanol / methanol / SDS, and are prepared into weakly alkaline isotonic solutions, which can kill the bacteria in the sample, but will cause damage to the cell structure, resulting in Cell contents are released and cannot be used for immunological or other methods of detection

At the same time, methanol\guanidine isothiocyanate in the preservation solution is volatile or highly toxic, and it is easy to cause volatilization during use and cause harm to operators

[0003] The existing preservation solution is inactivated with high-purity ethanol; some bacteria will have a stress response to high-concentration ethanol, resulting in a reduced inactivation effect; it contains protective nucleic acid components, but a large amount of surfactant in the preservation solution can also cause cell rupture , causing nucleic acid etc. to be in an exposed environment and easily degraded and destroyed; some inactivators are highly toxic, and operational errors during use will cause poisoning to users

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