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Chicken HS6ST3 gene 43bp indel polymorphic marker, application thereof, detection primer and reagent kit

A technology for polymorphism markers and detection primers, applied in the field of molecular genetics breeding, can solve the problem of no gene polymorphism, etc., and achieve the effects of easy popularization, strong operability, and sensitive detection

Inactive Publication Date: 2018-09-14
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few studies on the growth and carcass traits of HS6ST3 gene in livestock and poultry, and there is no related report on gene polymorphism in chickens

Method used

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  • Chicken HS6ST3 gene 43bp indel polymorphic marker, application thereof, detection primer and reagent kit
  • Chicken HS6ST3 gene 43bp indel polymorphic marker, application thereof, detection primer and reagent kit
  • Chicken HS6ST3 gene 43bp indel polymorphic marker, application thereof, detection primer and reagent kit

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Experimental program
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Effect test

Embodiment 1

[0034]In the present embodiment, the 43bp indel polymorphism marker of the first intron of the chicken HS6ST3 gene has a nucleotide sequence as shown in SEQ ID NO.1, and the 176-218 position from the 5' end is inserted or deleted.

[0035] The 43bp deletion in the polymorphic marker occurred between the 12076 and 12077 sites of the first intron of the HS6ST3 gene, and the NCBI GenBank accession number of the gene sequence was NC_006088. The discovery of the mutation site was the result of the previous analysis of the whole genome resequencing data of 5 Xichuan black-bone chickens, which further verified the 5 individuals. Subsequently, this experiment was carried out in large-scale populations of different breeds.

Embodiment 2

[0037] The detection primers of the first intron 43bp indel polymorphism marker of the chicken HS6ST3 gene in this embodiment are as follows:

[0038] Upstream primer P-F: 5'-CTGTGGTTCCCATGTCACTC-3';

[0039] Downstream primer P-R: 5'-AGTTAGCAAACCTGAAGAGGGAG-3'.

Embodiment 3

[0041] In this example, the detection kit for the 43bp indel polymorphism marker of the first intron of the chicken HS6ST3 gene includes the primers shown in Example 2, dNTPs, PCR reaction buffer, and DNA polymerase.

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Abstract

The invention relates to a chicken HS6ST3 gene 43bp indel polymorphic marker, application thereof, a detection primer and a reagent kit, and belongs to the technical field of molecular genetic breeding. The chicken HS6ST3 gene 43bp indel polymorphic marker, the application, the detection primer and the reagent kit have the advantages that the 43bp indel polymorphic marker in first introns of HS6ST3 genes is discovered, is obviously related to diversified economic characters such as 12-week shank circumferences and 6-week body weights of chicken and can be used for assisted selection and molecular breeding on the chicken; particularly, an amplification fragment designed according to a sequence shown as SEQ ID NO.1 contains the primer at 176th-218th bits from a 5' end of the sequence, whether the 176th-218th bits are inserted / deleted can be judged according to the sizes of amplification products, breeding on dominant genotype individuals with 176th-218th bit homozygous deletion can be reinforced, accordingly, the economic benefits of indigenous and commercial chicken can be increased, methods are free of enzyme digestion, accurate in genotype judgment, easy to operate, low in cost and short in period, and discrimination can be facilitated.

Description

technical field [0001] The invention relates to a chicken HS6ST3 gene 43bp indel polymorphic marker and application thereof, detection primers and a kit, belonging to the technical field of molecular genetics breeding. Background technique [0002] At present, with the breeding of livestock and poultry growth and feed efficiency, the production performance of livestock and poultry has been greatly improved. The meat quality of livestock and poultry meets the needs of human beings for stable protein intake and has become the material basis necessary for human life. . However, the unfavorable selection of poultry will lead to the loss of some dominant genotypes, while some dominant genotypes are still retained in low-selected local chickens. Therefore, on the premise of ensuring the high-quality traits of local germplasm resources, we can improve the growth rate and egg production rate of local chickens, and further protect, develop and utilize genetic resources of livestock ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 韩瑞丽李转见王香南康相涛郭亚苹李东华田亚东刘小军闫峰宾蒋瑞瑞李红李国喜孙桂荣王彦彬陈悦
Owner HENAN AGRICULTURAL UNIVERSITY
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