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Method for influencing zebrafish lateral hair cell regeneration by Wnt/beta-catenin signaling pathway

A signal pathway, hair cell technology, applied in biochemical equipment and methods, pharmaceutical formulations, biological particle analysis, etc.

Active Publication Date: 2018-09-14
HANGZHOU NORMAL UNIVERSITY
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However, the ototoxicity of cisplatin compounds is also obvious to all

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  • Method for influencing zebrafish lateral hair cell regeneration by Wnt/beta-catenin signaling pathway
  • Method for influencing zebrafish lateral hair cell regeneration by Wnt/beta-catenin signaling pathway
  • Method for influencing zebrafish lateral hair cell regeneration by Wnt/beta-catenin signaling pathway

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[0021]A method for the Wnt / β-catenin signaling pathway to affect the regeneration of zebrafish lateral line hair cells. The method uses flow cytometry to sort zebrafish lateral line neuromast helper cells, fluorescent quantitative PCR, whole embryo in situ hybridization, and exogenous addition of Wnt / β-catenin signaling pathway small-molecule compound inhibitors and enhancers were used to observe the spatiotemporal expression of various factors of Wnt / β-catenin signaling pathway and hair cell regeneration in the process of cisplatin-induced hair cell injury, and the model animal zebra Lateral line hair cell regeneration in fish; we used the model vertebrate zebrafish lateral line hair cell regeneration system to establish a cisplatin-induced hair cell injury and regeneration system, and used flow cytometry to sort lateral line neuromast synergistic cells and whole embryo in situ hybridization To explore the spatiotemporal expression changes of Wnt / β-catenin signal in the early...

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Abstract

The invention discloses a method for influencing zebrafish lateral hair cell regeneration by a Wnt / beta-catenin signaling pathway. The method comprises the steps of flow cytometric sorting of zebrafish lateral neuromast help cells, FQ-PCR, whole mount in situ hybridization and exogenous adding of a Wnt / beta-catenin signaling pathway small molecule compound inhibitor and enhancer, so as to observethe spatiotemporal expression of factors of the Wnt / beta-catenin signaling pathway and hair cell regeneration during cisplatin-induced hair cell injury, and influence the lateral hair cell regeneration of zebrafish (a model animal). In the cisplatin-induced hair cell dying process, the expression level of the Wnt / beta-catenin signaling factors is increased, including that the expression levels ofligand wnt2 and wnt3a and a key factor ctnnb1 in a lateral neuromast help cell group are increased; the expression levels of dkk1a and dkk2 in the help cell group are decreased, indicating the activation of the Wnt / beta-catenin signaling pathway during the hair cell dying process.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for Wnt / β-catenin signaling pathway to affect the regeneration of zebrafish lateral line hair cells. Background technique [0002] The Wnt / β-catenin signaling pathway, namely the Wingless-Int (Wnt) / β-catenin signaling pathway, is a recognized regulator of cell proliferation and differentiation. In the Wnt / β-catenin signaling pathway, after Wnts ligand binds to Frizzled receptors and Lrp5 / Lrp6 co-receptors, it activates the receptor complex by recruiting Dishevelled and depolymerizes the β-catenin degradation complex (GSK3β plays a major regulatory role). Finally, β-catenin accumulates in the cytoplasm and enters the nucleus to activate the expression of downstream factors together with TCF / LEF. Kawakami et al. found that inhibition of the Wnt / β-catenin signaling pathway leads to limb regeneration disorders in vertebrates such as zebrafish, axolotl, Xenopus laevis an...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/18A61P27/16C12Q1/6888C12Q1/686G01N15/00G01N15/10G01N15/14
CPCA61K45/00A61P27/16G01N15/00G01N15/10G01N15/14A61K31/18C12Q1/686C12Q1/6888G01N2015/1006G01N2015/1028G01N15/01G01N15/149C12Q2563/107C12Q2545/114
Inventor 芈肖肖
Owner HANGZHOU NORMAL UNIVERSITY
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