A kind of method of field herbaceous plant environmental control test
A herbal, controlled experiment technology, applied in the field of plant physiology and ecology research, can solve the problems of death, missed, difficult to control the gradient change of environmental factors, and achieve the effect of avoiding cross-contamination and ensuring accuracy.
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Embodiment 1
[0035] (1) Selection of sample plots for planting: in the desert areas where the native distribution of Huachai is selected, the area with flat terrain and little difference in soil background value is selected (24×14=336m 2 ), as a salt gradient test site;
[0036] Three gradients (mild salinization, moderate salinization, and severe salinization, set according to Table 1) were adopted. Select 6 plots of 6m×6m (Huahuachai is a perennial herb), with a buffer zone of at least 2m between each plot, one of which is a reserved test area, and each of the other 5 plots It is further divided into 4 plots, namely "control area", "mild salinization area", "moderate salinization area" and "severe salinization area", that is, there are 20 plots in total. Partitions (depth 1m) are placed around the plot to block the lateral exchange of salt and water, and to prevent the generation of cloned plants from affecting the experimental results. The sample setting is as follows: figure 1 , im...
Embodiment 2
[0050] (1) Selection of sample plots for planting: In desert areas where camel thorns are originally distributed, select areas with flat terrain and little difference in soil background values (34*22=748m 2 ), as the water gradient test site; for the isosmotic potential test corresponding to the salt gradient (recorded as PEG-light, PEG-medium, PEG-heavy), the reagent is PEG solution, and six 10m×10m (camel thorns are selected as semi-shrub), the other methods are the same as the salt gradient group, and the sample setting is as follows figure 2 , Figure 4 , Figure 6 .
[0051] (2) the plant selection of planting: dry environment plant selects camel thorn, selects robust 2-3 year old seedling stock, seedling height 5-7cm, root length 20-30cm, plant spacing 1.5m, pit diameter 60cm, pit depth 70cm, will Xerophytes were planted in the pit, and the seedlings were backfilled with soil to a distance of 10 cm from the pit mouth.
[0052] (3) In the first ten days of April of...
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