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A kind of composite bacterial agent and its method for preparing tobacco planting organic fertilizer

A compound bacterial agent and organic fertilizer technology, applied in the preparation of organic fertilizers, organic fertilizers, methods based on microorganisms, etc., can solve problems such as odor pollution, the impact of sustainable development of farms, and environmental pollution, and achieve the promotion of release, Improve disease resistance and improve the effect of disease resistance

Active Publication Date: 2020-07-14
上海晨耘农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term storage of pig manure not only attracts mosquitoes and flies, but also causes great pollution to the surrounding environment and groundwater sources. severe impact

Method used

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  • A kind of composite bacterial agent and its method for preparing tobacco planting organic fertilizer
  • A kind of composite bacterial agent and its method for preparing tobacco planting organic fertilizer
  • A kind of composite bacterial agent and its method for preparing tobacco planting organic fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Strain selection:

[0031] The active ingredients are calculated by weight ratio and include: 4 parts of Bacillus subtilis, 4 parts of Bacillus amyloliquefaciens, 2 parts of Thiobacillus oxidans, 2 parts of Leptospira ferrooxidans, 2 parts of Rhodotorula and 5 parts of Geotrichum mould.

[0032] 1) On the special medium for 6 kinds of bacteria, continuously streak culture, and select the bacteria with plump colony shape and the largest colony radius for species preservation and production. This process is operated continuously for more than 10 times until the performance of the strain is stable and the reproduction is about 1000 generations.

[0033] 2) The stress resistance of the screened strains is re-screened, and good growth can be ensured on a nutrient-poor plate.

[0034] 3) The strains were re-screened three times under simulated soil conditions. After sampling three kinds of soil to sterilize the soil, inoculate the mixed bacterial solution, continuous cultur...

Embodiment 2

[0038] The re-screened strains were cultured as follows:

[0039] (1), the compound bacterial agent is cultivated in the primary fermentation bacterium liquid medium, and the primary fermentation bacterium liquid medium is arrayed according to mass parts: 1000 parts of distilled water, 20 parts of glucose, 15 parts of peptone, 5 parts of sodium chloride and 0.5 part beef extract;

[0040] (2), then enter the secondary fermentation broth culture medium to obtain the secondary fermentation seed liquid, the secondary fermentation broth medium is composed of: 10000 parts of corn flour or soybean meal, 5000 parts of fish meal, 8000 parts of glucose and soybean meal 2000 parts of skin powder.

[0041] (3) Three-stage continuous solid fermentation: The second-stage fermented seed liquid is passed through a high-pressure spray device to spray the high-temperature steam detoxified solid fermentation material, and then enters the composting reactor, and the aeration time is once every ...

Embodiment 3

[0046] The application test of the special organic fertilizer for tobacco of the present invention.

[0047] Tobacco variety: K326;

[0048] Soil for testing: beef liver soil field;

[0049] Test scheme: This test selects two treatments, the organic fertilizer sample prepared by the present invention and the comparative example. According to the same principle of total nitrogen consumption, the comparative example is pure application of chemical fertilizers, and the nitrogen application rate per mu is 3.5kg, while the examples are 5%, 10%, 20%, 30% organic nitrogen plus 30% chemical fertilizer nitrogen. The test results are shown in Table 3.

[0050]Table 3: Application test results

[0051]

[0052] The test results show that the organic fertilizer of the present invention is superior to chemical fertilizers in the above indicators, and the application of the organic fertilizer of the present invention has increased the yield, quality and output value of tobacco leaves ...

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Abstract

The invention belongs to the field of microecological preparations and the technical field of organic fertilizer production, and particularly relates to a composite fungicide and a method for preparing tobacco planting organic fertilizer with the composite fungicide. The composite fungicide is prepared from bacillus subtilis, bacillus amyloliquefaciens, thiobacillus thiooxidans, leptospirillum ferrooxidans, rhodotorula and geotrichum mould. The composite fungicide is cultured in a primary zymophyte liquid culture medium and then enters a secondary zymophyte liquid culture medium to be cultured, secondary fermentation seed liquid is obtained, the secondary fermentation seed liquid is sprayed to solid fermentation materials obtained after high-temperature steam detoxification through a high-pressure spraying device, then the secondary fermentation seed liquid enters a compost reactor, and the aeration time interval is 2 hours each time. Pig manure and a mixture of corn flour, rape seed cakes and peanut shells are mixed to be used, the water content of the pig manure is precisely defined, no water needs to be additionally replenished in the whole fermentation process, the preparationprocess and operation are simplified, and uncertainty of irregularity of water replenishing operation to the biotransformation effect is also reduced.

Description

technical field [0001] The invention belongs to the field of micro-ecological preparations and the technical field of organic fertilizer production, and specifically relates to a compound bacterial agent and a method for preparing organic fertilizer for tobacco planting. Background technique [0002] The technology of producing organic fertilizer by solid fermentation is quite mature at home and abroad, but the commonly used strains are backward, and the growth rate of the strains is slow, resulting in a fermentation time of up to 5-6 days, which seriously affects production. Moreover, the selected microorganisms cannot achieve the effect of comprehensively improving the soil, which often causes excessive acidification of the soil, and the heavy metal secondary pollution caused by a large amount of animal manure is used and other serious problems. Therefore, it is urgent to find suitable strains through basic research and carry out scientific proportioning to comprehensively...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/16C05F17/20C05G3/80C12R1/125C12R1/07C12R1/01C12R1/645
CPCC05F3/00C05F17/00C05G3/00C05G3/80C12N1/14C12N1/16C12N1/20C05F5/002C05F11/00C05F11/08Y02W30/40
Inventor 万小平
Owner 上海晨耘农业科技有限公司
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