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Cell culture solution and establishment method of human primary lung cancer cell line

A technology for cell culture and establishment methods, which is applied in the field of cell culture medium and the establishment of primary human lung cancer cell lines. Short-term, growth-promoting effect

Inactive Publication Date: 2018-09-04
深圳市因诺转化医学研究院 +1
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  • Abstract
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Problems solved by technology

However, a general cell culture method for lung cancer tissue samples has not been established
And there is no simple and effective solution for the removal of fibroblasts, the main contaminating component in the culture process.

Method used

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  • Cell culture solution and establishment method of human primary lung cancer cell line
  • Cell culture solution and establishment method of human primary lung cancer cell line

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Embodiment Construction

[0015] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0016] In order to culture primary human lung cancer cells, it is necessary to configure a specific cell culture medium, referred to as F medium, for the cultivation of primary human lung cancer cells. The culture solution comprises a mixed medium in which F-12 medium and DMEM medium are mixed in a ratio of (3:1, v / v), and 5% (v / v) fetal bovine serum is added to the mixed medium, 2mM GlμtaMAX, 5μg / mL insulin, 0.4μg / mL hydrocortisone, 10ng / mL epidermal growth factor...

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Abstract

The invention discloses a cell culture solution. The cell culture solution is characterized in that fetal bovine serum, kinase inhibitor Y-27632, GlutaMAX, insulin, hydrocortisone, epidermal growth factors and adenine are added into an F-12 medium and DMEM mixture. By using the F culture solution, cell attachment and growth in tissue blocks can be promoted, and meanwhile the growth of fibroblastscan be promoted; by adopting the cell culture method, the human primary lung cancer cells can be established with a high success rate, the culture time is short, and multiple times of passage and proliferation of the cells can be realized. An important material source is provided for basic research in lung cancer and related translational medicine application. The cell culture solution has great practical significance and practical value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cell culture solution and a method for establishing human primary lung cancer cell lines. Background technique [0002] In the current tumor cell culture technology, the most commonly used technology for primary culture and line establishment of tumor cells is to apply basal medium supplemented with 10% fetal calf serum (FCS for short), such as RPMI-1640, to culture tumor cells . However, the current application of this tumor cell culture technology to cultivate primary tumor cells has a very low success rate. For example, the success rate of using this medium to cultivate primary lung cancer and prostate cancer cells is lower than 10%, and the success rate of subculture and line establishment is even lower. Therefore, currently established cell lines are mostly used in tumor research. Since most of the established cell lines have been cultured and passaged in vitro for a long t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2500/40C12N2501/11C12N2501/33C12N2501/39C12N2501/405
Inventor 王明军陈磊
Owner 深圳市因诺转化医学研究院
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