Method for cultivation of plants using metal nanoparticles and nutrient medium for its implementation
A technology of nano-particles and cultivation methods, which is applied in the biological and nano-fields of plant cultivation, can solve problems such as destroying cells, and achieve the effect of improving morphology
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[0044] For the preparation of the nutrient medium according to the invention, any known formulation of a balanced nutrient medium (containing all organic and inorganic macronutrients and micronutrients essential for plants) can be used as a basis. Therefore, in order to prepare the nutrient medium according to the present invention, the iron salts, zinc salts or copper salts or these salts are replaced by charge-neutral iron nanoparticles, charge-neutral zinc nanoparticles or charge-neutral copper nanoparticles, or combinations thereof, respectively. A combination of salts was introduced into the medium while the other components of the nutrient medium used as the base remained unchanged.
[0045] In order to demonstrate the possibility of implementing the present invention, the agar Murashing-Skoog nutrient medium formulation (see Table 1) was used as the basis to prepare the nutrient medium composition according to the embodiment of the present invention.
[0046] In an embo...
Embodiment 1
[0099] Example 1: Effect of metal nanoparticles and chitosan in nutrient medium composition on germination of tomato seeds.
[0100] Seed germination was characterized by the value of the germination index, defined as the percentage of the number of germinated seeds relative to the total number of seeds taken. Seed germination index was determined on day 15 after initiation of plant culture.
[0101] Figures 2A-2DShown is the effect of metal nanoparticles instead of metal salts and chitosan supplementation (100 mg / L) in the nutrient medium composition on the germination of Venice tomato seeds.
[0102] Figure 2A It was shown that the germination index of tomato seeds increased by 13-27% (compared to the control group) on the nutrient medium containing iron nanoparticles at a concentration of 0.6-10.0 mg / L. Addition of chitosan to nutrient media containing iron nanoparticles showed an additional 23-33% increase in germination index.
[0103] Such as Figure 2B It was s...
Embodiment 2
[0106] Example 2: Effect of Metal Nanoparticles in Nutrient Medium Compositions on Morphological Indicators of Pepper and Tomato Plants ring.
[0107] The morphological indicators of the plants (germination length, root length and green part biomass yield) were measured from the 40th day after the start of the cultivation of the plants on the nutrient medium containing metal nanoparticles (under the above conditions). The results are expressed as percentage magnitudes between the indices obtained in the experiment (test group) and in the control group.
[0108] Figure 3A-3D The effect of metal nanoparticles in the nutrient medium composition on the root length of LJ-king pepper is shown.
[0109] From Figure 3A As can be seen in , compared to the control group, in the nutrient containing iron nanoparticles at concentrations of 0.06 mg / L, 0.3 mg / L and 3.0 mg / L instead of iron in ionic form (5.6 mg / L as metal) Cultivation of pepper on medium resulted in increases in roo...
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