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Large-scale fusarium graminearum inoculation method

A wheat scab fungus and inoculum technology, applied in the directions of biochemical equipment and methods, horticultural methods, and botanical equipment and methods, can solve the problem of large-scale inoculation identification of unfavorable breeding materials, low degree of light simplification, and low inoculation efficiency. and other problems, to achieve the effect of low cost, high degree of light simplification, and high inoculation efficiency

Inactive Publication Date: 2018-08-31
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] To sum up, the problems existing in the existing technology are: time-consuming and labor-intensive, high input cost, low inoculation efficiency, low degree of simplification in production and application, and not conducive to large-scale inoculation identification of breeding materials

Method used

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  • Large-scale fusarium graminearum inoculation method
  • Large-scale fusarium graminearum inoculation method
  • Large-scale fusarium graminearum inoculation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Wheat variety: Chuanmai 104 (the main variety in Sichuan)

[0043] Preparation of conidia inoculum of Fusarium rubella wheat: Select 5-6 representative strong pathogenic strains as strains, and activate and culture these strains on PDA plates respectively. Adopt puncher to get 5 pieces of bacterium cakes from above-mentioned activated culture medium, inoculate in the CMC liquid medium of 250ml, each bacterial strain inoculates 11 bottles. On a constant temperature shaker at 25°C, 175rpm, culture for 6 days. Use freshly sterilized four-layer gauze to filter the liquid culture, collect the spore suspensions of each strain separately, mix them in equal proportions, shake them well and take samples, and adjust the concentration of conidia to 3×10 with a hemocytometer under a microscope. 5 individual / mL, as an inoculum for later use. If you do not inoculate temporarily, you can store the inoculum suspension in a refrigerator at 4°C for 5-6 days.

[0044] The formula of th...

Embodiment 2

[0055] A method for large-scale inoculation of wheat scab, comprising the following steps:

[0056] Step 1. Preparation of conidia inoculum of Fusarium rubella wheat: 5 representative strains with strong pathogenicity were selected as strains, and these strains were respectively activated and cultivated on PDA plates. Adopt puncher to get 6 pieces of bacterium cakes from above-mentioned activated culture medium, inoculate in the CMC liquid medium of 250ml, each bacterial strain inoculates 10 bottles. On a constant temperature shaker at 25° C., 175 rpm, culture for 7 days. Use freshly sterilized four-layer gauze to filter the liquid culture, collect the spore suspensions of each strain separately, mix them in equal proportions, shake them well and take samples, and adjust the concentration of conidia to 1×10 with a hemocytometer under a microscope. 5 Individual / mL Individual / mL, as an inoculum for later use. If you do not inoculate temporarily, you can store the inoculum susp...

Embodiment 3

[0062] A method for large-scale inoculation of wheat scab, comprising the following steps:

[0063] Step 1. Preparation of conidia inoculum of Fusarium rubella wheat: 6 representative strains with strong pathogenicity were selected as strains, and these strains were respectively activated and cultured on PDA plates. Adopt puncher to get 4 bacterium cakes from above-mentioned activated culture medium, inoculate in the CMC liquid medium of 250ml, each bacterial strain inoculates 12 bottles. On a constant temperature shaker at 25°C, 175rpm, culture for 5 days. Use freshly sterilized four-layer gauze to filter the liquid culture, collect the spore suspensions of each strain separately, mix them in equal proportions, shake them well and take samples, and adjust the concentration of conidia to 5×10 with a hemocytometer under a microscope. 5 individual / mL, as an inoculum for later use. If you do not inoculate temporarily, you can store the inoculum suspension in a refrigerator at 4...

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Abstract

The invention discloses a large-scale fusarium graminearum inoculation method which comprises the following steps of: preparing a fusarium graminearum conidium inoculant, adding the conidium inoculantinto tween 20 at nightfall or in a cloudy day when a material is identified at an early flowering period, uniformly spraying onto wheatears in a test plot to be subjected to inoculation identification through an electric sprayer, covering the wheatears with a white transparent plastic film, and removing the film after moisturizing. According to the inoculation method, a disease index and a prevalence rate of the ears reach 80-90% and 90-100% respectively under the film covering and moisturizing conditions.

Description

technical field [0001] The invention belongs to the technical field of plant pathogenic fungus control, and in particular relates to a large-scale inoculation method of wheat scab. Background technique [0002] Wheat scab is one of the major wheat diseases worldwide caused by Fusarium graminearum (Gibberella zeae (Schw.) Petch). The middle and lower reaches of the Yangtze River and the Northeast wheat region are traditionally endemic and high-incidence areas of wheat scab in my country. In recent years, with global climate warming and changes in farming systems and methods such as straw returning to the field, the occurrence range of scab has continued to expand to the Huanghuai wheat region, the frequency has continued to increase, and the damage has gradually increased. Fusarium head blight mainly damages the ears of wheat, and can infect and damage wheat at all stages of growth, especially in the flowering stage. When the weather conditions are suitable for the infectio...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G7/06C12R1/645
CPCA01G7/06C12N1/14
Inventor 徐志张重梅于文娟张姝杨芳谢家廉姬红丽彭云良
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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