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A preparation method and detection method of a composite material biosensor for detecting aflatoxin b1

An aflatoxin and biosensor technology, which is applied in the analysis of materials, material analysis by electromagnetic means, instruments, etc., can solve the problems of contact with a large number of toxic and harmful substances, cumbersome operation steps, and increased cumbersomeness. Short analysis time, effect of increased current response

Active Publication Date: 2020-07-14
武汉睿奇生物工程有限公司
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Problems solved by technology

[0004] High-performance liquid chromatography has the advantages of good selectivity, high sensitivity, and strong signal. However, due to the fluorescence quenching of aflatoxin B1 and G1 in contact with water, appropriate derivatization reactions need to be carried out before detection, so it is increased The cumbersomeness of sample pretreatment
[0005] The equipment and reagents used in thin-layer chromatography are simple and low-cost, and can carry out quantitative and semi-quantitative analysis of aflatoxins in samples, but the operation is cumbersome, the detection limit is high, and the experimenters need to be exposed to a large number of toxic and harmful substances
[0006] Fluorescence spectrophotometry has good accuracy and precision, but it is not widely used in the laboratory because it is mostly used to detect the total amount of aflatoxin and the operation steps are cumbersome

Method used

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  • A preparation method and detection method of a composite material biosensor for detecting aflatoxin b1

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Embodiment

[0029] Example: see attached figure 1 , a method for preparing a composite material biosensor for detecting aflatoxin B1.

[0030] The steps are:

[0031] (1) Preparation of silver nanoparticles, using high temperature citric acid to reduce silver nitrate to prepare silver nanoparticles:

[0032] Accurately weigh 18mg of silver nitrate and add it to a 250ml three-necked flask, add 98ml of deionized water, fix the condensation reflux device on the three-necked flask, heat in an oil bath, and stir for 800 rpm. Heat the oil bath to 180°C. When the solution in the flask boils, pipette 2ml of sodium citrate solution into the three-necked flask, continue to boil for 1 hour, stop heating, and cool to room temperature naturally. Transfer the prepared silver sol to a Erlenmeyer flask, seal it and store it away from light, and place it for more than 150 hours for later use.

[0033] (2) Preparation of graphene oxide-nanometer silver composite material:

[0034] Under the condition o...

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Abstract

The invention discloses a preparation method of a composite material biological sensor for detecting aflatoxin B1 and a detection method thereof. The sensor is prepared from a graphene oxide-nanosilver-aflatoxin B1 monoclonal antibody composite material. The preparation method has the advantages that the specificity combining principle of aflatoxin B1 monoclonal antibody and antigen is utilized todetect; compared with the nanogold, the cost of the nanosilver in experiment is low; compared with the high performance liquid chromatography method, the instrument price is low; the operation is simple and convenient, the analysis time is short, the detection cost is low, the aflatoxin B1 can be qualitatively and quantitatively detected, the minimum detection limit can reach 7.19*10<-10>mu g / kg,and a good linear relationship is formed within the range of 7.19*10<-10> to 2.47*10<-4>mu g / kg; the reaction is sensitive, and the increasing of current response without adding the signal amplifyingmatters of chitosan, potassium ferricyanide and the like in the experiment is realized.

Description

technical field [0001] The invention belongs to the field of biosensor preparation, in particular to a preparation method and a detection method of a composite material biosensor for detecting aflatoxin B1. Background technique [0002] Aflatoxin is a secondary metabolite mainly produced by Aspergillus flavus and Aspergillus parasiticus. It is a type of mycotoxin with the most stable physical and chemical properties found in nature. It is the most toxic mycotoxin and extremely harmful to human health. A class of mycotoxins with strong toxicity, carcinogenicity, teratogenicity and mutagenicity. It widely exists in moldy peanuts, rice, corn and other crops and related foods. Naturally occurring aflatoxins are divided into four types according to their chemical structures: B1, B2, G1, and G2. Among them, aflatoxin B1 is the most toxic, 10 times that of potassium cyanide and 68 times that of arsenic. Aspergillus toxin B1 is one of the mandatory inspection items for most foods....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/308G01N27/3277G01N27/3278
Inventor 傅小红徐坤郭柯宇刘先科卿三琳雷怡尚书勇梅丽常波张强
Owner 武汉睿奇生物工程有限公司
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