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Application of macrocarpal I to preparation of anti-tumor drug

An anti-tumor drug, the technology of large fruit eucalyptal, applied in the field of medicine, can solve problems such as limited effect

Active Publication Date: 2018-08-28
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many targeted therapy drugs targeting traditional drug targets, but few are used in metastatic colorectal cancer, and the effect is very limited

Method used

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  • Application of macrocarpal I to preparation of anti-tumor drug
  • Application of macrocarpal I to preparation of anti-tumor drug
  • Application of macrocarpal I to preparation of anti-tumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The technical scheme of the present invention will be further explained below in conjunction with experiments.

[0026] Cell culture

[0027] Colorectal cancer cell lines are derived from cell lines already in our laboratory. The medium and fetal bovine serum used for cell culture were purchased from Gibico. The cell line is cultured in RPMI-1640 medium containing 10% fetal bovine serum, and the culture environment is 37℃, 5% CO 2 In the incubator, take cells in good growth state for subsequent experiments.

[0028] Cell proliferation experiment-concentration gradient

[0029] Eucalyptol I was dissolved in DMSO to prepare a mother liquor of 0.1μmol / μl. When colorectal cancer RKO and HCT116 cells grow to 80% confluence, 0.25% trypsin digestion, DMEM complete medium resuspended, counted, inoculated in a 96-well plate, 7×10 per well 3 Cells. After the cell monolayer adheres, eucalyptus macrocarpa I is added to make the final concentration 20, 50, 80, 100, 200, 300 μM, respectiv...

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Abstract

The invention discloses application of macrocarpal I to preparation of an anti-tumor drug. In an experiment, the inventor finds that the macrocarpal I can effectively inhibit proliferation in vitro orinvasion of colorectal cancer cells, can inhibit the clonality of the colorectal cancer cells, can reduce the growth rate of the subcutaneous tumor of a naked mouse and has certain specificity to thecolorectal cancer cells. In addition, the macrocarpal I is extremely high in safety and expected to be a natural anti-tumor micro-molecular compound applied to the treatment of the metastatic colorectal cancer.

Description

Technical field [0001] The invention belongs to the field of medicine, and relates to new applications of existing compounds, and specifically relates to the application of Eucalyptus macrocarpa I in the preparation of anti-tumor, especially colorectal cancer drugs. Background technique [0002] Colorectal cancer is a malignant tumor of the digestive tract that is very harmful to humans, and its incidence has been increasing year by year in recent years. The early symptoms of colorectal cancer are not obvious. Many patients have metastases before radical surgery. Metastasis is an important factor affecting the survival and prognosis of colorectal cancer patients. To find drug targets related to colorectal cancer metastasis, based on drug targets Screening effective targeted therapy drugs is the key to the treatment of colorectal cancer. At this stage, the main treatment for most tumors is still surgical resection, radiotherapy and chemotherapy. Surgical resection in the early s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/11A61P35/00A61P35/04
CPCA61K31/11A61P35/00A61P35/04
Inventor 齐鲁丁彦青
Owner SOUTHERN MEDICAL UNIVERSITY
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