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Rapid detection reagent for nitrite in food, kit and corresponding detection method

A nitrite and detection reagent technology, applied in the field of food quality and safety detection, can solve the problems of expensive equipment, complicated operation, and threats to the health of the people, and achieve the effect of cheap reagents and simple operation

Inactive Publication Date: 2018-08-21
智锐达仪器科技南通有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, some unscrupulous vendors use excessive nitrite, which seriously threatens the health of the people, and ion chromatography and other instruments and equipment are expensive, complicated to operate, and require strict professional skills of operators. Therefore, a fast, Accurate and easy-to-operate nitrite detection method is particularly important

Method used

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  • Rapid detection reagent for nitrite in food, kit and corresponding detection method
  • Rapid detection reagent for nitrite in food, kit and corresponding detection method
  • Rapid detection reagent for nitrite in food, kit and corresponding detection method

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Embodiment Construction

[0021] The rapid detection method for nitrite in food of the present invention, the specific detection steps include:

[0022] 1) Reagent preparation

[0023] Reagent A: 4g / L sodium p-aminobenzenesulfonate solution, weigh 0.4g sodium p-aminobenzenesulfonate, dissolve in 20% hydrochloric acid solution, and dilute to 100ml;

[0024] Reagent B: 2g / L naphthaleneethylenediamine hydrochloride, weigh 0.2g naphthaleneethylenediamine hydrochloride, dissolve it in water, and dilute to 100ml.

[0025] 2) Sample processing: Weigh 10g of the food to be tested, chop it or break it with a blender, weigh 1g with a balance into the sample cup, add 20ml of pure water, and soak for 10~15min.

[0026] 3) Take 2ml of the soaking solution (if it is turbid, decolorize or filter it), add 100μl of reagent A, let stand for 3 minutes, add 100μl of reagent B, mix well and react for 10 minutes, ready to be tested.

[0027] 4) Zero calibration and detection of the sample to be tested: turn on the multi-p...

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Abstract

The invention discloses a rapid detection reagent for nitrite in food, a kit and a corresponding detection method. The reagent comprises a reagent A and a reagent B, wherein the reagent A is a sodiumsulfanilate solution, and the reagent B is an N-ethylenediamine dihydrochloride solution. After the novel sodium sulfanilate solution is adopted as the reagent A, nitrite in the food can be rapidly and accurately detected, the detection process is simple to operate, and the reagent is low in cost. The sodium sulfanilate solution has the linear dependence of a standard curve superior to that of sulfanilic acid while relative to a sulfanilic acid solution, so that the reagent is suitable for automatic detection and interpretation of a multi-parameter analyzer.

Description

technical field [0001] The invention specifically relates to a rapid detection reagent, kit and corresponding detection method for nitrite in food, belonging to the technical field of food quality and safety detection. Background technique [0002] Nitrite mainly refers to sodium nitrite and potassium nitrite, which are allowed to be used in limited quantities as coloring agents in meat products. At the same time, nitrates rich in vegetables and meat will also be converted into nitrites under the action of reductase in the case of improper pickling, storage or processing. [0003] Nitrite is a highly toxic substance. If an adult ingests 0.2-0.5 grams, it can cause poisoning, and 3 grams can cause death. Nitrite can oxidize human hemoglobin and lose the ability to transport oxygen, causing chronic and acute poisoning. It can also react with secondary amine compounds in food and in the human body to form highly carcinogenic nitrosamine compounds. [0004] However, some unscr...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 葛明娟杨燕李乾
Owner 智锐达仪器科技南通有限公司
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