Genetic identification and molecular marker-assisted breeding method for rice amylose content QTL site qSAC3

Abstract

The invention discloses a genetic identification and molecular marker-assisted breeding method for QTL site qSAC3 capable of stably improving the rice amylose content under various environments. In the prior art, the rice amylose content is affected by the environment, and especially the amylose content of the japonica rice filled at the high temperature is significantly decreased. The present invention discloses the interval of the QTL site qSAC3 capable of stably improving the rice amylose content under different environments, and a plurality of polymorphic molecular markers Y6665, Y7237, Y8113 and Y8212 positioned in the interval and used for assisted selection. According to the present invention, the qSAC3 of the Indica rice 9311 is introduced into the japonica rice Nipponbare genome through advanced generation backcross by using the relevant molecular marker-assisted selection to obtain the chromosome fragment replacement line; and the amylose content of the japonica rice is significantly decreased at the high temperature, such that the site qSAC3 derived from the Indica rice can be used for improving the quality of conventional breeding rice, and has important application value in the cultivation of high-quality japonica rice varieties capable of resisting high temperature environment.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding. Specifically, the invention relates to a genetic identification and molecular marker-assisted breeding method capable of stably increasing rice amylose content QTL site qSAC3 under various environments. Background technique [0002] Improving rice quality is one of the important goals of rice breeding, but rice quality is not only affected by genetic material, but also by various environmental factors, especially environmental temperature. It is very important to analyze the variation law of rice quality under different environments, and analyze the relationship between genetic action sites controlling rice quality and environmental temperature, so as to provide theoretical guidance for people to breed high-quality rice varieties adapted to different environments. [0003] Rice amylose content (AC) is one of the important indicators to evaluate the eating quality of rice. Genetic studie...

AI Technical Summary

Problems solved by technology

However, so far, the relevant research results are still relatively small, which is not conducive to in-depth analysis of the influence of environmental temperature on the genetic mechanism of rice AC, and is far from meeting the needs of high-quality rice variety breeding

Images