Genetic Identification and Marker-Assisted Breeding of Amylose Content Qtl Locus Qsac3 in Rice

Abstract

The invention discloses a method for genetic identification and molecular marker-assisted breeding that can stably increase the QTL site qSAC3 of rice amylose content under various environments. The amylose content of rice is affected by the environment, especially the amylose content of japonica rice that is filled at high temperature is significantly reduced. The invention discloses the location of a QTL site qSAC3 that can stably increase the amylose content of rice under different environments and Among them, multiple polymorphic molecular markers Y6665, Y7237, Y8113 and Y8212 can be used for auxiliary selection. The qSAC3 of indica rice 9311 was introduced into the genome of japonica rice Nipponbare through high-generation backcrossing and marker-assisted selection to obtain chromosome segment replacement lines. Since the amylose content of japonica rice will decrease significantly at high temperature, the qSAC3 locus derived from indica rice can not only be used to improve the quality of conventional breeding rice, but also has important applications in the process of cultivating high-quality japonica rice varieties that are resistant to high temperature environments value.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding. Specifically, the invention relates to a genetic identification and molecular marker-assisted breeding method capable of stably increasing rice amylose content QTL site qSAC3 under various environments. Background technique [0002] Improving rice quality is one of the important goals of rice breeding, but rice quality is not only affected by genetic material, but also by various environmental factors, especially environmental temperature. It is very important to analyze the variation law of rice quality under different environments, and analyze the relationship between genetic action sites controlling rice quality and environmental temperature, so as to provide theoretical guidance for people to breed high-quality rice varieties adapted to different environments. [0003] Rice amylose content (AC) is one of the important indicators to evaluate the eating quality of rice. Genetic studie...

AI Technical Summary

Problems solved by technology

However, so far, the relevant research results are still relatively small, which is not conducive to in-depth analysis of the influence of environmental temperature on the genetic mechanism of rice AC, and is far from meeting the needs of high-quality rice variety breeding

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