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Method for treating aquaculture wastewater through immobilized chlorella

A technology for immobilizing small balls and aquaculture wastewater, applied to chemical instruments and methods, fixed on/in organic carriers, non-polluting water treatment, etc., can solve problems such as large footprint, poor mechanical strength, and difficulty in separation problems, achieve good nitrogen and phosphorus removal effects, reduce costs, and avoid toxic effects

Inactive Publication Date: 2018-08-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of microalgae to treat wastewater also has some disadvantages: large area, difficult to separate, serious cell loss, etc.
Existing microalgae immobilization methods include the use of materials such as sponges, pebbles, and gravels for biosorption immobilization, which has a poor immobilization effect; polyvinyl alcohol is used as the immobilization material, but polyvinyl alcohol has certain biological toxicity; Although chitosan immobilized microalgae has a certain effect, the immobilized pellets made of chitosan have poor mechanical strength and the pellets are easily biodegraded

Method used

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  • Method for treating aquaculture wastewater through immobilized chlorella
  • Method for treating aquaculture wastewater through immobilized chlorella
  • Method for treating aquaculture wastewater through immobilized chlorella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Sodium alginate, agar and PVA three immobilized materials to the situation of denitrification and dephosphorization of aquaculture wastewater

[0054] Sodium alginate (Alginate), agar (Agar) and PVA are three immobilized materials to embed Chlorella, and the method is shown in 2. Put the immobilized chlorella made of equal volumes of 2% sodium alginate, agar and PVA algae liquid mixture into 500mL aquaculture wastewater respectively, and place the Erlenmeyer flask in a constant temperature light incubator. The culture conditions are 25°C, 4000 lux light, and 12h:12h light-to-dark ratio. Shake the Erlenmeyer flask 5 times a day and take samples regularly.

[0055] Determination of NH in water samples 4 + -N, NO 3 - -N, NO 2 - -N, TN and TP. figure 1 , Sodium alginate, agar and PVA immobilized materials on the NH of aquaculture wastewater 4 + -N Remove case. The three kinds of immobilized chlorella have a certain removal effect on ammonia nitrogen in...

Embodiment 2

[0060] Embodiment 2: The situation of denitrification and dephosphorization of aquaculture wastewater by immobilized chlorella and free chlorella

[0061] Centrifuge the chlorella solution cultivated to the logarithmic phase at 4000r / min for 5 minutes, wash with deionized water three times and then centrifuge, take an appropriate volume of microalgae solution and add it to 500mL aquaculture wastewater, adjust the algae density (OD) to about 0.3, and then Take an equal volume of algae liquid and use the above-mentioned 2 immobilization methods to embed the chlorella with 2% sodium alginate to make immobilized chlorella. In addition, set a blank water sample group and a blank immobilized ball group. Place the Erlenmeyer flask in a constant temperature light incubator. The culture conditions are 25°C, 4000 lux light, and 12h:12h light-to-dark ratio. Shake the Erlenmeyer flask 5 times a day and take samples regularly.

[0062] Determination of NH in water samples 4 + -N, NO ...

Embodiment 3

[0066] Example 3: The situation of nitrogen and phosphorus removal from aquaculture wastewater with different sodium alginate contents

[0067] Centrifuge the chlorella liquid cultured to the logarithmic phase at 4000r / min for 5 minutes, wash with deionized water three times and then centrifuge, and use sodium alginate solutions with concentrations of 1%, 2%, 3%, and 4% according to the method in 2 Made into immobilized pellets. Immobilized chlorella with different concentrations of sodium alginate were put into 500 mL of aquaculture wastewater (labeled 1%, 2%, 3%, 4%) respectively, and the Erlenmeyer flasks were placed in a constant temperature light incubator. The culture conditions are 25°C, 4000 lux light, and 12h:12h light-to-dark ratio. Shake the Erlenmeyer flask 5 times a day and take samples regularly.

[0068] Determination of NH in water samples 4 + -N, NO 3 - -N, TN and TP. Figure 10 , the effect of different sodium alginate contents on the NH of aquaculture...

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Abstract

The invention discloses a method for treating aquaculture wastewater through immobilized chlorella and belongs to the field of water purification. By utilizing absorption and utilization of chlorellaon nutritive materials and other trace elements in the wastewater and immobilization on sodium alga acid, alga-water separation can be well performed when the chlorella achieves an effect of purifyingthe wastewater. The method has the advantages that the aquaculture wastewater is treated by chlorella immobilized by the sodium alga acid, and increasingly severe fresh water pressure is relieved; moreover, due to absorption and utilization of the chlorella on various elements in the wastewater, the wastewater is purified, and the load of the wastewater discharged into natural water finally is further retarded; in addition, after the chlorella is immobilized, the alga-water separation can be well realized, and equipment cost is reduced.

Description

technical field [0001] The invention relates to a method for treating culture wastewater with immobilized chlorella, belonging to the field of water body purification. Background technique [0002] With the development of the economy, people's demand for aquatic products is increasing day by day. When the catch cannot meet the market demand, the aquaculture industry has developed rapidly. However, the situation of ecological imbalance, environmental deterioration and resource shrinkage caused by extensive management and resource-dependent aquaculture has been very serious at home and abroad. The original extensive aquaculture method directly discharged the aquaculture wastewater without treatment, which aggravated the degree of eutrophication and water quality pollution in the adjacent waters of the aquaculture area, and caused a series of harmful ecological and environmental problems. [0003] The main pollutants in aquaculture wastewater are ammonia nitrogen, nitrite, org...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C02F3/32C02F103/02C02F101/16C02F101/10
CPCC02F3/322C02F2101/105C02F2101/16C02F2101/166C02F2103/20C12N11/04C12N11/10
Inventor 任洪艳陈红芬阮文权
Owner JIANGNAN UNIV
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