Technical method for high-efficiency cultivation of arabidopsis thaliana
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A technology of Arabidopsis thaliana and technology, applied in the field of biotechnology research, can solve the problems of strict environmental factor requirements, difficult to meet Arabidopsis thaliana, dwarf plants, etc., and achieve the effects of low technical requirements, rich and comprehensive nutrients, and robust plants
Inactive Publication Date: 2018-08-21
贵州省油菜研究所
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Problems solved by technology
However, the current Arabidopsis culture process has short plants, strict requirements on environmental factors, and fewer inflorescences, which is difficult to meet the needs of Arabidopsis genetic transformation experiments.
Method used
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Embodiment 1
[0019] 1. On February 10, 2017, weigh 5 kg of Brassica napus rapeseed cake and put it in a glass container, add 1.25 kg of water and mix well, and place it in a greenhouse at about 20°C for 10 days of fermentation.
[0020] 2. On February 16, 2017, col ecotype Arabidopsis thaliana seeds were sterilized with 0.1% HgCl2, rinsed with water, sterilized with 75% alcohol for 3 times, washed with sterilized water for 2 times, and then sterilized at 4 ℃ refrigerator for vernalization for 4 days.
[0021] 3. On February 21, 2017, weigh 10 kg of nutrient soil and 5 kg of vermiculite, mix the fermented rapeseed cake: nutrient soil: vermiculite in a ratio of 1:2:1, and place it in the autoclave The pot is sterilized under high temperature and high pressure, and after sterilization, it is placed at room temperature to cool down and then packed in flower pots.
[0022] 4. The Arabidopsis seeds treated with vernalization in step 2 are evenly sown in the pot prepared in step 3, placed in the...
Embodiment 2
[0025] 1. On March 15, 2017, weigh 5 kg of mustard-type rapeseed cake and put it in a glass container, add 1.25 kg of tap water, and place it in a greenhouse at about 20°C for 10 days of fermentation.
[0026] 2. On March 21, 2017, the RLD ecotype Arabidopsis thaliana seeds were sterilized with 0.1% HgCl2, rinsed with water for 3 times, sterilized with 75% alcohol for 3 times, and then washed with sterilized water After the second time, the vernalization treatment was carried out in a refrigerator at a temperature of 4°C for 4 days.
[0027] 3. On March 26, 2017, the balance weighed 10 kg of nutrient soil and 5 kg of vermiculite, mixed the fermented rapeseed cake: nutrient soil: vermiculite in a ratio of 1:2:1, and placed it in an autoclave The fungus pot is sterilized by high temperature and high pressure. After sterilization, it is placed at room temperature to cool down and then packed in flower pots.
[0028] 4. On March 26, 2017, the Arabidopsis thaliana seeds that had b...
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Abstract
The invention discloses a technical method for high-efficiency cultivation of arabidopsis thaliana. The technical method comprises the following steps: fermenting rapeseed cakes for 10 days, mixing the fermented rapeseed cakes with arabidopsis thaliana culture nutrient soil and vermiculite, carrying out high-temperature high-pressure disinfection and sterilization in a high-pressure sterilizationpot, carrying out placing for cooling at room temperature, then sowing vernalized arabidopsis thaliana seeds, removing main inflorescence to inhibit the apical dominance when the arabidopsis thalianagrows to a squaring stage, and applying the arabidopsis thaliana to a transformation test when the arabidopsis thaliana grows to a full-bloom stage. The fermented rapeseed cakes are added in the arabidopsis thaliana culture medium nutrient soil and the vermiculite, so that the nutrients required by the arabidopsis thaliana growth are more abundant and comprehensive, the growth is more vigorous, and the arabidopsis thaliana is more robust. In the squaring stage, the apical dominance is effectively inhibited through the removal of the main inflorescence, and the plants grow well, so that more inflorescence is produced, and the transformation test of the arabidopsis thaliana is facilitated. The operation process is simple, the technical requirement degree is low, the environmental factors arenot strictly required, and the cultivation method can be carried out in a general illumination incubator.
Description
technical field [0001] The invention relates to the field of biotechnology research, in particular to a technical method for high-efficiency cultivation of Arabidopsis thaliana. technical background [0002] Arabidopsis thaliana belongs to the Brassicaceae plant and is the most common model plant, which is widely used in various fields of genetics, physiology, biochemistry and molecular biology research. The plant height of Arabidopsis is relatively short, only about 30cm; the genome is simple, with only 5 pairs of chromosomes. Although the genome is simple, it has relatively complex genomic information of Brassicaceae with high homology; the growth cycle of Arabidopsis is short, The next generation of Arabidopsis seeds can be harvested in only about 6 weeks; the morphological characteristics are simple, but it has complete roots, stems, leaves, flowers, fruits and common tissues and organs of seeds; the whole genome sequencing has been completed, based on the above reasons,...
Claims
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