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A kind of decalcification solution for fish histopathological section

A technique of histopathology and slicing, applied in the field of tissue decalcification, which can solve the problems of slow decalcification time and poor transparency of decalcification solution, and achieve the effects of shortening decalcification time, small molecular weight, and reducing the degree of acid hydrolysis

Active Publication Date: 2020-12-29
SICHUAN AGRI UNIV
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  • Abstract
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Problems solved by technology

[0005] Aiming at the above-mentioned prior art, the present invention provides a decalcification solution for fish histopathological sections to solve the technical problems of slow decalcification time and poor transparency of the existing decalcification solution

Method used

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  • A kind of decalcification solution for fish histopathological section

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Embodiment 1 Embodiment 3

[0029] In Examples 1 to 3, the formula of the decalcification solution is moderate, and the color of the tissue after decalcification is normal, and the transparency is good, which facilitates the observation of the internal structure. Embodiment four Compared with embodiment three, sodium chloride increases, and decalcification time shortens, and this is because sodium chloride can significantly increase solution ionic strength as strong electrolyte, and according to the salt effect of insoluble electrolyte, the increase of ionic strength in solution It can reduce the chance of the combination of positive and negative ions to form precipitates, increase the solubility of poorly soluble electrolytes, and improve the decalcification efficiency. Compared with Example 2, Example 5 lacks a tissue clearing agent. After decalcification, the tissue is slightly whitish and poor in transparency, which seriously affects the observation of the internal morphology and structure. Example 6...

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Abstract

The invention discloses a fish tissue pathological slice decalcifying fluid, which comprises following components: formaldehyde (4-5 v / v%), methanol (50-60 v / v%), formic acid (10-15 v / v%), hydrochloric acid (15-20 v / v%), a tissue clearing reagent (5-6 v / v%), sodium chloride (1-2 v / v%), and the balance being distilled water; wherein the tissue clearing agent comprises following components: 50 to 55vol.% of dimethyl sulfoxide, 15 to 20 mass% of fructose, 1 to 2 mass% of sodium bicarbonate, 10 to 15 mass% of sodium dodecyl sulfonate, and the balance being water. The provided decalcifying fluid can solve the technical problems that in the prior art, the decalcification time is long and the transparency is bad.

Description

technical field [0001] The invention belongs to the technical field of tissue decalcification, and in particular relates to a decalcification liquid for fish histopathological slices. Background technique [0002] The skeleton of a fish is the main structure that supports the body and protects the soft organs. Bone tissue is a hard knotted tissue composed of bone cells and bone matrix. The bone matrix contains a large amount of inorganic salts, mainly hydroxyapatite-calcium phosphate and calcium carbonate. Due to the high hardness of bone tissue, it is easy to detach when slicing. Therefore, in the process of slice making, especially fish brain, gill, and muscle tissue slices, decalcification solution is usually used for decalcification treatment. Its main function is to expand the hoof tissue and soften the bone tissue to facilitate the preparation of stained slices. [0003] At present, the commercially available decalcification solution used to process fish tissue is ex...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/2813G01N1/30G01N2001/305
Inventor 黄小丽冯杨段靖汪开毓邓永强杨世勇耿毅欧阳萍陈德芳
Owner SICHUAN AGRI UNIV
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