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Method for increasing content of astaxanthin in haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high price and harmful substance pollution

Active Publication Date: 2018-08-17
北京耕天下生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although synthetic astaxanthin has been approved by the US FDA as a food additive, it is expensive and may be contaminated by other harmful substances. The product also contains isomers with unnatural structures

Method used

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  • Method for increasing content of astaxanthin in haematococcus pluvialis
  • Method for increasing content of astaxanthin in haematococcus pluvialis
  • Method for increasing content of astaxanthin in haematococcus pluvialis

Examples

Experimental program
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Embodiment 1

[0030] A columnar glass photobioreactor (height 50 cm, inner diameter 6 cm) and BG11 medium were used for autotrophic culture indoors to obtain the culture solution of Haematococcus pluvialis in the swimming stage. The algal species is Haematococcus pluvialis, which comes from the freshwater algae species bank of the Institute of Hydrobiology, Chinese Academy of Sciences, number 712. Astaxanthin induction was performed in the same reactor, and the culture medium was diluted with nitrogen-deficient BG11 medium so that the initial cell concentration in the induction phase was 0.2 g (dry weight) / L. When preparing the medium, MEA was added to a final concentration of 100 mg / L, and the medium was sterilized at 121 °C and 0.1 MPa for 20 minutes, and then diluted after cooling. Aeration stones commonly used in this field (pore size 30-60 μm) are used for aeration and mixing, and 8 LED light bars provide a light intensity of 200 μmol / (m 2 s) white light illumination. Use an air cond...

Embodiment 2

[0034] Others are the same as in Example 1, except that MEA is added to the culture solution of Haematococcus pluvialis to a final concentration of 200 mg / L.

[0035] MEA with a final concentration of 200 mg / L was added to the culture medium of Haematococcus pluvialis, and the batch induction culture was carried out for 8 days. The same microalgae were cultured under the same culture conditions, except that MEA was not added. It took 12 days for the cells to turn red completely, and the astaxanthin content accounted for 0.52% of the dry weight of the cells. In comparison, the astaxanthin content increased by 63 % after adding 200 mg / LMEA. Such as figure 1 shown.

[0036] MEA at a final concentration of 200 mg / L was added to the culture medium of Haematococcus pluvialis, and the batch induction culture was carried out for 8 days, and the cell concentration was 0.170 g (dry weight) / L; while using the same reactor and the same culture conditions The same microalgae were cultu...

Embodiment 3

[0038] Others are the same as in Example 1, the difference is the source of Haematococcus pluvialis culture solution and the way of adding ethanolamine in the induction stage. Specifically, a closed columnar glass photobioreactor (height 50 cm, inner diameter 6 cm) and BG11 medium plus 1 g / L sodium zirconate was used as the medium for mixed culture to obtain the swimming stage of P. Coccus culture medium; Astaxanthin was induced in the same reactor, and the culture medium was diluted with nitrogen-deficient BG11 medium so that the initial cell concentration in the induction phase was 0.2 g (dry weight) / L. On the first day and the fourth day of culture, 50 mg / L MEA was added to the final concentration of 100 mg / L, and the added MEA solution was sterilized by ultraviolet disinfection.

[0039] Add twice 50 mg / L to the final concentration of 100 mg / L of MEA in the Haematococcus pluvialis culture medium, batch induction culture for 8 days, the ratio of astaxanthin to cell dry weig...

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Abstract

The invention discloses a method for increasing the content of astaxanthin in haematococcus pluvialis. The method comprises the step of adding monoethanolamine (MEA) into a culture solution at an immobile stage of culture of haematococcus pluvialis until the final concentration is 50-400 mg / L. The method for increasing the content of astaxanthin in haematococcus pluvialis is a new technical routefor increasing the content of astaxanthin in haematococcus pluvialis, the content of astaxanthin in cells is significantly increased, the culture time is shortened, and the culture efficiency is improved.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology and relates to a method for increasing the astaxanthin content in Haematococcus pluvialis, that is, a method for increasing the astaxanthin content in Haematococcus pluvialis by adding ethanolamine. Background technique [0002] Astaxanthin (3,3'-dihydroxy-β,β'-carotene-4,4'-dione) belongs to carotenoids and is the main pigment of crustaceans such as shrimp, crab and salmon. Strong anti-oxidation function, can remove free radicals produced by ultraviolet radiation in the body, regulate and reduce these photochemical damages, and have a good therapeutic effect on skin cancer caused by ultraviolet rays. In addition, astaxanthin can also significantly promote the production of lymph node antibodies, especially the production of antibodies to antigens associated with T cells in the body. Although artificially synthesized astaxanthin has been approved by the US FDA as a food additive, it is...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12N1/12C12N1/38C12R1/89
CPCC12N1/12C12N1/38C12P23/00
Inventor 孙中亮孙利芹王自清王书亚
Owner 北京耕天下生物科技有限公司
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