Nucleic acid extraction method

Abstract

The invention discloses a nucleic acid extraction method. The nucleic acid extraction method comprises the steps of cracking and transferring. In the cracking step, thermal cracking of nucleic acid iscompleted, and magnetic beads adsorb nucleic acid released in the thermal cracking process, and the cracking step is completed in a cracking area; the magnetic beads adsorbing the nucleic acid are transferred into pre-treatment fluid, and the transferring step is completed; and the pre-treatment fluid is located in a pre-treatment area, and the cracking area is mutually independent of the pre-treatment area. The nucleic acid extraction method has the advantages that the nucleic acid extraction steps are simplified, the extraction period is shortened, the nucleic acid extraction success rate is improved, and loss of the nucleic acid extraction amount is less.

Description

technical field [0001] The present invention relates to the field of nucleic acid extraction, more specifically, to a nucleic acid extraction method. Background technique [0002] The extraction of nucleic acid, such as DNA or RNA, currently mainly adopts two extraction methods: spin column method and magnetic bead method. The traditional spin column technology also uses membranes for nucleic acid purification. The principle of its membrane belongs to the silicon adsorption method. This membrane only has a strong affinity and adsorption force for nucleic acids. During the operation, the sample was firstly lysed by protease at a temperature of 56 °C, and then the lysing solution was added to the column for centrifugation, the nucleic acid was adsorbed on the membrane, and other impurities were thrown out, and finally the nucleic acid was eluted with the eluent. This method has good extraction effect, but requires heating during lysis, and multiple centrifugations during nucl...

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Problems solved by technology

[0012] The existing problems of the traditional magnetic rod extraction method are: 1. The purified nucleic acid eluate needs to be transferred manually, and manual operation errors are prone to occur during the transfer process, resulting in contamination by human factors and false positives

2. After salt washing, washing, and elution, there are many washing and elution steps, which take a long time, and the error rate will be greatly increased, especially when a large number of samples need to be processed in a short period of time, the cross-contamination is serious and the cost is high. More manpower, and the error rate will increase significantly

3. The multiple transfers of magnetic beads lead to inevitable damage to the nucleic acid content. This is because the magnetic beads are exposed, and the nucleic acids adsorbed on the magnetic beads are exposed to the outside, and are susceptible to impurities in the environment, such as dust, enzymes, or other substances. Material contamination, especially for RNA, is more susceptible to degradation

4. Nucleic acid undergoes repeated washing, elution and heating, which may significantly reduce the integrity of nucleic acid and intensify the fragmentation of nucleic acid, which is not conducive to subsequent processing, such as subsequent sequencing, amplification or other utilization

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