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Nucleic acid field rapid-extraction tube and use method thereof

A nucleic acid extraction device and nucleic acid technology, applied in biochemical equipment and methods, specific-purpose bioreactors/fermenters, biomass post-processing, etc., can solve the complex structure of nucleic acid extraction equipment, inapplicable on-site diagnosis, and diagnosis cycle Long and other problems, to achieve the effect of simple structure, easy operation, and reduce nucleic acid loss

Active Publication Date: 2019-03-12
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Claims
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Problems solved by technology

[0017] In order to solve the problems existing in the prior art of nucleic acid extraction equipment such as complex structure, easy to cause cross-contamination, long diagnostic cycle, high price, and unsuitable for on-site diagnosis of animal epidemics, on the one hand, the present invention provides a nucleic acid extraction device , including: a push rod, a hollow main body and a nucleic acid adsorption device, wherein the push rod is nested in the main body; at least one liquid storage chamber arranged independently of the main body, wherein the liquid storage chamber communicates with the main body; a control valve, wherein , the control valve is set to control the communication and / or non-communication state between the liquid storage chamber and the main body

Method used

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  • Nucleic acid field rapid-extraction tube and use method thereof
  • Nucleic acid field rapid-extraction tube and use method thereof
  • Nucleic acid field rapid-extraction tube and use method thereof

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Embodiment Construction

[0056] In order to illustrate the overall concept of the present invention more clearly, the following will be described in detail in the form of embodiments in conjunction with the accompanying drawings. In the following description, numerous specific details are given in order to provide a more thorough understanding of the present application. It will be apparent, however, to one skilled in the art that the present application may be practiced without one or more of these details. In other examples, some technical features known in the art are not described in order to avoid confusion with the present application.

[0057] Such as figure 1 and figure 2 As shown, the present embodiment provides a nucleic acid extraction device, comprising: a hollow main body 3, a push rod 1 with a rubber plug 2 at the bottom nested inside the main body 3, an adsorption device arranged at the bottom of the main body 3, In this embodiment, the adsorption device is an adsorption tube 4 . W...

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Abstract

The invention provides a nucleic acid field rapid-extraction tube and a use method thereof. The nucleic acid field rapid-extraction tube comprises a push rod, a hollow main tube body and a nucleic acid adsorption device, wherein the push rod is nested in the main tube body; at least one liquid storage chamber is arranged independently of the main body, wherein the liquid storage chamber is communicated with the main tube body; a control valve is arranged to control a communication and / or non-communication state between the liquid storage chamber and the main tube body. The nucleic acid extraction device provided by the invention is simple and portable, can complete a process from tissue sample treatment to rapid extraction and purification of the nucleic acid within 20 to 30 minutes, and is particularly suitable for initially screening a large number of samples rapidly in clinical first-line detection for farms, ports and the like, where epidemics easily occurs, and meanwhile the independently formed liquid storage chamber can avoid cross-infection of reagents or the samples, thus being beneficial to improvement of the purity of nucleic acid.

Description

technical field [0001] The invention relates to the field of molecular biology instruments and equipment, in particular to a nucleic acid on-site rapid extraction tube and a method for using the same. Background technique [0002] Generally speaking, nucleic acid extraction requires multi-step work. First, biological sample materials such as cells and tissue materials need to be crushed, nucleases are inactivated, nucleic acids are released, and other tissue or cell components such as proteins, polysaccharides, and lipids are removed. Thereby obtaining high quality nucleic acid. [0003] Since Swiss physician Friedrich Miescher first successfully extracted DNA from cells in 1869 to the 1990s, nucleic acid extraction has been a tedious, time-consuming task requiring the use of toxic reagents. Until recent years, with the emergence of solid-phase adsorption technology and the development of biochemistry, this problem has been greatly alleviated. However, the rapid extraction...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N15/10C12Q1/6806
CPCC12N15/101C12Q1/6806
Inventor 袁向芬吴绍强吕继洲林祥梅
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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