Small molecular weight silkworm pupa collagen
A technology of collagen and small molecular weight, applied in the field of small molecular weight silkworm chrysalis collagen, to achieve the effect of ensuring stability, high yield and increasing added value
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Embodiment 1
[0023] A small molecular weight silkworm chrysalis collagen with a molecular weight of 600-1000Da. The collagen is rich in nutrition, and the amino acid ratio of silkworm chrysalis protein is excellent. It can improve the activity of lymphocytes, improve the body's immune function through the lymphatic system, and enhance the body's disease prevention and disease resistance. , can also scavenge free radicals in organisms and promote blood cell regeneration in the body, and has significant anti-tumor activity, and can be used as a nutritional additive in bread, noodles, biscuits, condiments or high-protein beverages.
[0024] A preparation method of silkworm chrysalis collagen with small molecular weight, which specifically comprises the following steps:
[0025] 1) Degreasing: add degreasing agent to silkworm chrysalis powder according to the ratio of material to liquid 1:6 (g / mL), then degrease at 35°C for 30 minutes, filter with suction after cooling, the filter residue is de...
Embodiment 2
[0034] A silkworm chrysalis collagen with small molecular weight, the molecular weight is 600-1000Da.
[0035] A preparation method of silkworm chrysalis collagen with small molecular weight, which specifically comprises the following steps:
[0036] 1) Degreasing: add degreasing agent to silkworm chrysalis powder according to the material-liquid ratio of 1:4 (g / mL), then degrease at 45°C for 20 minutes, suction filter after cooling, the filter residue is degreased silkworm chrysalis, and then bake at 70°C dry, spare;
[0037] 2) Enzymatic hydrolysis: add phosphate buffer solution with a pH of 8.5 to the defatted silkworm chrysalis at a material-to-liquid ratio of 1:20 (g / mL), then add 2% neutral protease by weight of the defatted silkworm chrysalis, and enzymatically hydrolyze at 55°C for 1 hour , then raise the temperature to 100°C, keep it warm for 10 minutes to inactivate the enzyme, and after cooling, centrifuge for 10 minutes in a centrifuge with a temperature of 5°C an...
Embodiment 3
[0045] A silkworm chrysalis collagen with small molecular weight, the molecular weight is 600-1000Da.
[0046] A preparation method of silkworm chrysalis collagen with small molecular weight, which specifically comprises the following steps:
[0047] 1) Degreasing: add degreasing agent to silkworm chrysalis powder according to the ratio of material to liquid 1:5 (g / mL), then degrease at 40°C for 25 minutes, filter after cooling, the filter residue is degreasing silkworm chrysalis, and then bake at 60°C dry, spare;
[0048] 2) Enzymatic hydrolysis: add phosphate buffer solution with pH 8.0 to the defatted silkworm chrysalis according to the ratio of material to liquid 1:25 (g / mL), then add 3% neutral protease by weight of the defatted silkworm chrysalis, and enzymatically hydrolyze 1.5 h, then raise the temperature to 95°C, keep it warm for 15 minutes to inactivate the enzyme, and after cooling, centrifuge in a centrifuge with a temperature of 2°C and a rotation speed of 9000r...
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