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Small molecular weight silkworm pupa collagen

A technology of collagen and small molecular weight, applied in the field of small molecular weight silkworm chrysalis collagen, to achieve the effect of ensuring stability, high yield and increasing added value

Inactive Publication Date: 2018-08-17
海盐县凌特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And prepare collagen with silkworm chrysalis, there is no report yet

Method used

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Experimental program
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Effect test

Embodiment 1

[0023] A small molecular weight silkworm chrysalis collagen with a molecular weight of 600-1000Da. The collagen is rich in nutrition, and the amino acid ratio of silkworm chrysalis protein is excellent. It can improve the activity of lymphocytes, improve the body's immune function through the lymphatic system, and enhance the body's disease prevention and disease resistance. , can also scavenge free radicals in organisms and promote blood cell regeneration in the body, and has significant anti-tumor activity, and can be used as a nutritional additive in bread, noodles, biscuits, condiments or high-protein beverages.

[0024] A preparation method of silkworm chrysalis collagen with small molecular weight, which specifically comprises the following steps:

[0025] 1) Degreasing: add degreasing agent to silkworm chrysalis powder according to the ratio of material to liquid 1:6 (g / mL), then degrease at 35°C for 30 minutes, filter with suction after cooling, the filter residue is de...

Embodiment 2

[0034] A silkworm chrysalis collagen with small molecular weight, the molecular weight is 600-1000Da.

[0035] A preparation method of silkworm chrysalis collagen with small molecular weight, which specifically comprises the following steps:

[0036] 1) Degreasing: add degreasing agent to silkworm chrysalis powder according to the material-liquid ratio of 1:4 (g / mL), then degrease at 45°C for 20 minutes, suction filter after cooling, the filter residue is degreased silkworm chrysalis, and then bake at 70°C dry, spare;

[0037] 2) Enzymatic hydrolysis: add phosphate buffer solution with a pH of 8.5 to the defatted silkworm chrysalis at a material-to-liquid ratio of 1:20 (g / mL), then add 2% neutral protease by weight of the defatted silkworm chrysalis, and enzymatically hydrolyze at 55°C for 1 hour , then raise the temperature to 100°C, keep it warm for 10 minutes to inactivate the enzyme, and after cooling, centrifuge for 10 minutes in a centrifuge with a temperature of 5°C an...

Embodiment 3

[0045] A silkworm chrysalis collagen with small molecular weight, the molecular weight is 600-1000Da.

[0046] A preparation method of silkworm chrysalis collagen with small molecular weight, which specifically comprises the following steps:

[0047] 1) Degreasing: add degreasing agent to silkworm chrysalis powder according to the ratio of material to liquid 1:5 (g / mL), then degrease at 40°C for 25 minutes, filter after cooling, the filter residue is degreasing silkworm chrysalis, and then bake at 60°C dry, spare;

[0048] 2) Enzymatic hydrolysis: add phosphate buffer solution with pH 8.0 to the defatted silkworm chrysalis according to the ratio of material to liquid 1:25 (g / mL), then add 3% neutral protease by weight of the defatted silkworm chrysalis, and enzymatically hydrolyze 1.5 h, then raise the temperature to 95°C, keep it warm for 15 minutes to inactivate the enzyme, and after cooling, centrifuge in a centrifuge with a temperature of 2°C and a rotation speed of 9000r...

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Abstract

The invention discloses a small molecular weight silkworm pupa collagen with a molecular weight of 600-1000Da. A preparation method of the collagen is as follows: adding a degreasing agent to silkwormpupa powder for degreasing, filtering by suction, and drying filter residue; adding a phosphate buffer liquid and neutral protease to the degreased silkworm pupa for enzymatic hydrolysis, performingenzyme deactivation, and centrifuging after cooling to obtain supernatant, namely a crude protein solution; adding a chitosan solution to the crude protein solution, stirring, centrifuging, putting supernatant into an ice water bath, adding ammonium sulfate, stirring until the ammonium sulfate is completely dissolved, standing overnight, centrifuging, dissolving a precipitate in a phosphate buffer, dialyzing, allowing dialysate into a hydroxyapatite column, adding the buffer to elute, and freezing-drying to obtain the small molecular weight silkworm pupa collagen. The beneficial effects are asfollows: the small molecular weight silkworm pupa collagen has high purity, high activity, and relatively-concentrated molecular weight, and the preparation method is simple and feasible, high in degreasing efficiency, high in yield, easy in industrial production and higher in safety and improves the added value of silkworm pupa.

Description

technical field [0001] The invention relates to the technical field of deep processing of silkworm chrysalis, in particular to a silkworm chrysalis collagen with small molecular weight. Background technique [0002] Collagen, or collagen, is a biological polymer synthesized by animal cells. It is widely found in animal bones, tendons, cartilage, skin and other connective tissues. It has the functions of supporting organs and protecting the body. 30% of protein. There are many kinds of aquatic animals in the ocean, and different aquatic animals have huge differences in their morphology and body structure. Squid, an invertebrate cephalopod, has a well-developed dermis, and the collagen in its body is mainly distributed in the dermis. Collagen has a stable molecular structure, low immunogenicity, and good biocompatibility. These properties determine its use. Collagen has a wide range of uses. Products have various shapes and uses, and are used in many fields such as food, che...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/36C07K1/34C07K1/30C07K1/16C12P21/06
CPCC07K14/78C12P21/06
Inventor 董珊珊
Owner 海盐县凌特生物科技有限公司
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