Antitumor application of apios americana flower ethanol extract
An ethanol extraction and anti-tumor technology, applied in the field of medicine, can solve the problem of rarely reported anti-tumor activity, and achieve the effects of significant anti-tumor activity, expanding application fields and good development prospects.
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Embodiment 1
[0047] Embodiment 1, the anti-tumor application of the ethanol extract of beetroot flower, such as Figure 1-Figure 8 As shown, the ethanol extract of taro flower has significant antitumor effect.
[0048] The specific preparation steps of the taro flower ethanol extract are as follows:
[0049] (1) According to the solid-liquid ratio of 1g: 10ml, after mixing the taro flower and the ethanol containing 0.1mol / L hydrochloric acid, it is processed and beaten by a juice extractor. The obtained slurry was ultrasonically leached for 90 minutes at 45° C., the extract was centrifuged at 3000 g for 30 minutes, and the extract was centrifuged to obtain a supernatant.
[0050] (2), replace the taro flower in step (1) with the centrifuged filter residue obtained in step (1), repeat the extraction 2 times, combine the filtrate three times, and concentrate by rotary evaporation to obtain a concentrated solution (concentrated by rotary evaporation to 15% of the original volume).
[0051] ...
Embodiment 2
[0062] Embodiment 2, the influence of the ethanol extract of japonica flower on the tumor volume of mice;
[0063] The mouse tumor volume (before putting to death) after intragastric gavage in the measurement embodiment 1 28 days, the result is as follows figure 2 As shown, the tumor volume in the control group was the largest. Compared with the control group, the positive control group and the intervention group with ethanol extracts of different concentrations of bean taro flowers had a certain inhibitory effect on the increase of tumor volume, and the tumor volume in the positive drug group was the smallest. The above results show that the ethanol extract of taro flower can alleviate the increase of tumor volume in tumor-bearing nude mice.
Embodiment 3
[0064] Embodiment 3, the influence of ethanol extract of taro flower on mouse tumor weight and tumor inhibition rate;
[0065] After 28 days of intragastric administration in Example 1, the mice were fasted for 12 hours, and the mice were killed by cervical dislocation after blood was collected from the orbits. According to the formula: tumor inhibition rate=(1-weight of tumor mass in experimental group / weight of tumor mass in control group)*100%. The result is as image 3 , Figure 4 As shown, the positive control group and the intervention groups of ethanol extracts of taro flower with different concentrations all had inhibitory effects on tumor growth, and the positive drug group had the highest tumor inhibition rate.
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