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Detection reagent for procalcitonin colloidal gold by immunoturbidimetry

An immunoturbidimetric and detection reagent technology, applied in the field of medicine, can solve problems such as pollution of the environment, and achieve the effects of no environmental pollution, long shelf life, and significant economic and social benefits.

Active Publication Date: 2018-08-03
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above situation, in order to overcome the defects of the prior art, the object of the present invention is to provide a procalcitonin colloidal gold immunoturbidimetric detection reagent, which has high accuracy and precision, good repeatability, simple operation and short measurement time. , Applicable to various types of automatic biochemical analyzers, and can effectively solve the problem of environmental pollution in practical applications

Method used

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  • Detection reagent for procalcitonin colloidal gold by immunoturbidimetry
  • Detection reagent for procalcitonin colloidal gold by immunoturbidimetry
  • Detection reagent for procalcitonin colloidal gold by immunoturbidimetry

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Embodiment 1

[0011] A procalcitonin colloidal gold immunoturbidimetric detection reagent of the present invention comprises reagent 1 and reagent 2, and described reagent 1 is made of trishydroxymethylaminomethane (Tris) 6.05g, sodium azide (NaN 3 ) 0.5g, add water to 1000ml and mix evenly, and adjust the pH value to 8.15 with hydrochloric acid to make; the reagent 2 is made of tris (Tris) 6.05g, bovine serum albumin (BSA) 1g, polyethylene Pyrrolidone K30 (PVP-K30) 0.5g, polyethylene glycol 20000 (PEG20000) 0.5g, Tween-20 (Tween-20) 2ml, sucrose 35g, sodium azide (NaN 3 ) 0.5g, casein 0.5g, add water to 1000ml and mix well, adjust the pH value to 8.15 with hydrochloric acid to obtain a buffer solution, then dissolve the colloidal gold particles labeled with anti-human procalcitonin antibody with the buffer solution, the ratio is 11 mg antibody / 1L colloidal gold solution, adjust the concentration to 520nm absorbance (OD) is 0.2, obtains reagent 2.

Embodiment 2

[0013] A procalcitonin colloidal gold immunoturbidimetric detection reagent of the present invention comprises reagent 1 and reagent 2, and described reagent 1 is made of trishydroxymethylaminomethane (Tris) 6.05g, sodium azide (NaN 3 ) 1.0g, add water to 1000ml and mix evenly, and adjust the pH value to 8.25 with hydrochloric acid to make; the reagent 2 is made of tris (Tris) 6.05g, bovine serum albumin (BSA) 5g, polyethylene Pyrrolidone K30 (PVP-K30) 1.0g, polyethylene glycol 20000 (PEG20000) 1.0g, Tween-20 (Tween-20) 5ml, sucrose 50g, sodium azide (NaN 3 ) 1.0g, casein 1.25g, add water to 1000ml and mix well, adjust the pH value to 8.25 with hydrochloric acid to obtain a buffer solution, then dissolve the colloidal gold particles labeled with anti-human procalcitonin antibody with the buffer solution, the ratio is 11 mg antibody / 1L colloidal gold solution, adjust the concentration to 520nm absorbance (OD) is 0.25, obtains reagent 2.

Embodiment 3

[0015] A procalcitonin colloidal gold immunoturbidimetric detection reagent of the present invention comprises reagent 1 and reagent 2, and described reagent 1 is made of trishydroxymethylaminomethane (Tris) 6.05g, sodium azide (NaN 3 ) 1.5g, add water to 1000ml and mix evenly, and adjust the pH value to 8.35 with hydrochloric acid to make; the reagent 2 is made of tris (Tris) 6.05g, bovine serum albumin (BSA) 10g, polyethylene Pyrrolidone K30 (PVP-K30) 1.5g, polyethylene glycol 20000 (PEG20000) 1.5g, Tween-20 (Tween-20) 10ml, sucrose 65g, sodium azide (NaN 3 ) 1.5g, casein 2g, add water to 1000ml and mix well, adjust the pH value to 8.35 with hydrochloric acid to obtain a buffer solution, then dissolve the colloidal gold particles labeled with anti-human procalcitonin antibody with the buffer solution, the ratio is 11 mg antibody / 1L colloidal gold solution, adjust the concentration to 520nm absorbance (OD) is 0.3, obtain reagent 2.

[0016] This operation method can be used...

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Abstract

The invention relates to a detection reagent for procalcitonin colloidal gold by immunoturbidimetry, which can effectively solve the detection problem of procalcitonin. A technical scheme is characterized that the detection reagent comprises a reagent 1 and a reagent 2, the reagent 1 is prepared by the following steps: water is added in tromethamine and sodium azide, the materials are uniformly mixed, and hydrochloric acid is used for adjusting a pH value; and the reagent 2 is prepared by the following steps: water is added in tromethamine, bovine serum albumin, polyvinylpyrrolidone, polyethylene glycol 20000, tween-20, cane sugar, sodium azide, and casein, the materials are uniformly mixed, and hydrochloric acid is used for adjusting the pH value to obtain a buffer solution, the buffer solution is used for dissolving anti-human procalcitonin antibody-labeled colloidal gold particles, concentration is adjusted, and the reagent 2 is obtained. The detection reagent has the advantages ofscientific component, novel and unique characteristics, easy preparation, low cost, stable performance, long shelf-life, accurate testing, no environmental pollution, and obvious economic and social benefits, and is an innovation for the procalcitonin detection reagent.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a procalcitonin colloidal gold immunoturbidimetric detection reagent. Background technique [0002] Procalcitonin (PCT) is a glycoprotein whose molecular structure is composed of 32 amino acids CT, 21 amino acids calstatin and N-terminal fragments containing 57 amino acids. The propeptide of cT is a glycoprotein with no hormone activity and an endogenous non-steroidal anti-inflammatory substance. It was discovered by Maya et al. in 1975, and its complete structure was determined in 1981. Assicot et al. found in 1993 that the increase of serum PCT level was closely related to systemic infectious diseases. Under normal physiological conditions, procalcitonin without hormonal activity is produced and secreted by thyroid C-cells, and is decomposed into calcitonin by special intracellular proteases, and PCT mRNA is also expressed in different tissues , as also expressed in liver, lung, kidne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/543
CPCG01N33/54313G01N33/74G01N2333/585
Inventor 王玉金刘丽杨书豪杜迅李珊珊胡宜亮孙晨阳张志龙李寒冰王佰涛张勇魏传军李志金
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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