Oocystis concentrate with high activity and high stress resistance, and preparation method and application thereof

Abstract

The invention specifically relates to an Oocystis concentrate with high activity and high stress resistance, and a preparation method and application thereof, belonging to the technical fields of aquaculture water quality regulation and water environment treatment. The preparation method comprises the following steps: isolation and cultivation of purebred Oocystis; seed selection of Oocystis withhigh activity and high stress resistance; enlarged cultivation; industrial cultivation; collection; concentration; and the like, wherein continuous aerated cultivation is carried out under the conditions of a temperature of 23 to 28 DEG C and an illumination of 1800 to 2000 Lx. Compared with the prior art, the concentration of the Oocystis concentrate of the invention can reach 130 to 150 million / ml, can be stored at normal temperature for 95-105 days, has a survival rate of 95% or more, and is convenient for storage, transportation and usage; and the Oocystis concentrate has high activity, high stress resistance and stable algal phase, can keep the water quality of a culture pond in benign equilibrium for a long time, is applicable to preparation of algal phase stabilizers for seawater orbrackish-water culture ponds of marine food products, and shows good industrial application prospects.

Description

technical field [0001] The invention relates to the technical field of aquaculture water quality regulation and control, in particular to a highly active and stress-resistant oocyst concentrate, a preparation method and application thereof. Background technique [0002] Oocystis belongs to Chlorophyta, Trebouxiophyceae, Chlorellales, Oocystaceae. The plant body is a spherical or elliptical spherical group, and the group is usually composed of 2, 4 or 8 cells, and there is a gelatinous coating composed of pectin outside the group. The algae cells are oval or oval, with a length of 10-19 μm and a width of 9-13 μm, and generally produce 2, 4, 8 or 16 spore-like spores. Oocysts are mainly distributed in water bodies rich in organic matter such as estuaries and aquaculture ponds. They have the characteristics of long duration and stable population. They can reduce the concentration of harmful factors such as ammonia nitrogen and nitrite in water bodies, and increase dissolved ox...

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Problems solved by technology

[0004] The traditional way of cultivating oocysts is to cultivate in open cement ponds, which has a large scale of cultivation and high yield, but the disadvantages are: (1) It is easy to pollute, occupies a large area, is greatly affected by the weather, is not easy to control, and the growth of algae cells is slow , the doubling time is long, the one-time investment is large and the output is low, and it is not suitable for the expansion stage; (2) the algal phase of the oocysts cultivated by the traditional method is not stable enough, and it is easy to age and lose algae. It is especially suitable for shrimp and other culture ponds In the middle and late stages of cultivation, the water quality of the pond will be unstable, and the algae phase will be unfavorable for the growth of prawns, and the algae phenomenon will occur, and the dead algae will produce a large amount of algae toxins, which will lead to the death of seafood such as prawns; (3) The cultivated oocysts are difficult to store and transport for a long time at room temperature, causing inconvenience to use

These make it difficult for oocysts to be applied on a large scale in actual production

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