Expanded culture method of oocystis algae species with high activity and strong resistance

Abstract

The invention relates to the technical field of aquaculture water quality regulation and control and water body environment treatment, in particular to an expanded culture method of oocystis algae species with high activity and strong resistance. The method comprises the steps of oocystis pure species separation, pure species cultivation, high-activity and strong-resistance oocystis breeding and secondary expansion culture, and continuous aeration culture is performed under the conditions that the temperature is 23-28 DEG C, and the illumination is 1800-2000 Lx. Compared with the prior art, the oocystis algae species prepared by the expanded culture method provided by the invention have high activity and strong resistance, are stable in algae growth state and capable of maintaining a healthy balanced state of water quality in an aquaculture pond for a long term and directly performing industrial culture, thereby having a very good industrial application prospect.

Description

technical field [0001] The invention relates to the technical field of aquaculture water quality control and treatment, in particular to a method for expanding the cultivation of highly active and highly stress-resistant oocyst algae species. Background technique [0002] Oocystis belongs to Chlorophyta, Trebouxiophyceae, Chlorellales, Oocystaceae. The plant body is a spherical or elliptical spherical group, and the group is usually composed of 2, 4 or 8 cells, and there is a gelatinous coating composed of pectin outside the group. The algae cells are oval or oval, with a length of 10-19 μm and a width of 9-13 μm, and generally produce 2, 4, 8 or 16 spore-like spores. Oocysts are mainly distributed in water bodies rich in organic matter such as estuaries and aquaculture ponds. They have the characteristics of long duration and stable population. They can reduce the concentration of harmful factors such as ammonia nitrogen and nitrite in water bodies, and increase dissolved ...

AI Technical Summary

Problems solved by technology

[0004] The traditional way of cultivating oocysts is to cultivate in open cement ponds, which has a large scale of cultivation and high yield, but the disadvantages are: (1) It is easy to pollute, occupies a large area, is greatly affected by the weather, is not easy to control, and the growth of algae cells is slow , the doubling time is long, the one-time investment is large and the output is low, and it is not suitable for the expansion stage; (2) the algal phase of the oocysts cultivated by the traditional method is not stable enough, and it is easy to age and lose algae. It is especially suitable for shrimp and other culture ponds In the middle and late stages of cultivation, the water quality of the pond will be unstable, and the algae phase will be unfavorable for the growth of prawns, and the algae phenomenon will occur, and the dead algae will produce a large amount of algae toxins, which will lead to the death of seafood such as prawns; (3) The cultivated oocysts are difficult to store and transport for a long time at room temperature, causing inconvenience to use

These make it difficult for oocysts to be applied on a large scale in actual production

Images