Preparation method of lentinan

A technology of lentinan and Lentinus edodes, which is applied in chemical instruments and methods, ultrafiltration, membrane technology and other directions, can solve the problems of low extraction rate of lentinan and low polysaccharide purity, and achieves high extraction rate, good pore connectivity and porosity. high effect

Active Publication Date: 2018-08-03
河南和生食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to address the deficiencies in the prior art, to provide a method for extracting lentinan, which solves the problems of low extraction rate of existing lentinan and low purity of the obtained polysaccharide, and achieves high-efficiency extraction of high-purity polysaccharides. Effect

Method used

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  • Preparation method of lentinan

Examples

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Effect test

Embodiment 1

[0029] A preparation method of lentinan, comprising the steps of:

[0030] 1) Pretreatment: take mushrooms, remove impurities, wash, drain, cut and pulverize to obtain superfine powder;

[0031] 2) Cell wall breaking and beating: the powder obtained in step 1) is first subjected to cell wall breaking treatment, and then sequentially added to acetone and ethanol for beating treatment at 0°C, and the solid-liquid ratio of the added acetone and ethanol to the powder is 1g / 5ml; static precipitation, filtration to separate the precipitate and supernatant; the method of cell wall breaking treatment is low-temperature micro-nano wall breaking technology, the powder is placed below -170°C to freeze, and then dissolved at room temperature, repeated Several times, so that the cell wall is broken; the ethanol is ethanol with a volume concentration of not less than 95%;

[0032] 3) Clean water extraction: add the precipitate obtained in step 2) into clean water, and stir to dissolve it c...

Embodiment 2

[0048] A preparation method of lentinan, comprising the steps of:

[0049] 1) Pretreatment: take mushrooms, remove impurities, wash, drain, cut and pulverize to obtain superfine powder;

[0050] 2) Cell wall breaking and beating: the powder obtained in step 1) is first subjected to cell wall breaking treatment, and then sequentially added to acetone and ethanol at 10°C for beating treatment, and the solid-liquid ratio of the added acetone and ethanol to the powder is 2g / 5ml; static precipitation, filtration to separate the precipitation and supernatant; the method of cell wall breaking treatment is low-temperature micro-nano wall breaking technology, the powder is placed below -170°C to freeze, and then dissolved at room temperature, repeated Several times, so that the cell wall is broken; the ethanol is ethanol with a volume concentration of not less than 95%;

[0051] 3) Clean water extraction: Add the precipitate obtained in step 2) into clean water, stir to dissolve it co...

Embodiment 3

[0067] A preparation method of lentinan, comprising the steps of:

[0068] 1) Pretreatment: take mushrooms, remove impurities, wash, drain, cut and pulverize to obtain superfine powder;

[0069] 2) Cell wall breaking and beating: the powder obtained in step 1) is first subjected to cell wall breaking treatment, and then sequentially added to acetone and ethanol at 5°C for beating treatment, and the solid-liquid ratio of the added acetone and ethanol to the powder is 1.5g / 5ml; static precipitation, filtration to separate the precipitate and supernatant; the method of cell wall breaking treatment is low-temperature micro-nano wall breaking technology, the powder is placed below -170°C to freeze, and then dissolved at room temperature, Repeat several times to break the cell wall; the ethanol is ethanol with a volume concentration of not less than 95%;

[0070] 3) Clean water extraction: add the precipitate obtained in step 2) into clean water, and stir to dissolve it completely;...

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Abstract

The invention discloses a preparation method of lentinan. The preparation method comprises the following steps: 1) pre-treating; 2) breaking cell walls and pulping; 3) extracting with clean water; 4)carrying out alcohol precipitation to obtain crude polysaccharide; 5) filling the crude polysaccharide on ion exchange resin and collecting an eluting solution to obtain a de-colored polysaccharide solution; 6) carrying out micro-filtration and ultra-filtration, and concentrating to obtain a concentrated solution; 7) freezing and drying the concentrated solution obtained by step 6) to obtain a lentinan product. According to the preparation method provided by the invention, the obtained lentinan product has high extraction rate and high purity; meanwhile, the lentinan has excellent pharmaceutical activity and prominent advantages.

Description

technical field [0001] The invention relates to the field of extraction of effective plant components, in particular to a method for extracting lentinan. Background technique [0002] Mushroom [Lentinus edodes (Berk.) Sing], also known as Xiangtan and thick mushroom, is a fungus of the Basidiomycetes Apiaceae family. It is known as the "king of mountain delicacies". It is a high-protein, Low-fat nutraceutical food. As early as in the "Compendium of Materia Medica", it is recorded that shiitake mushrooms are "flat in nature and sweet in taste, can benefit qi and relieve hunger, cure wind and blood, reduce phlegm and regulate qi, benefit taste and food, and manage urination." [0003] Lentinan (LNT for short) is an important effective medicinal component extracted from Lentinus edodes. It is a D-(1→3) glucose residue as the main chain and (1→6) glucose residue as side chains of dextran. Since 1969, Chihara et al. used hot water to extract and purify lentinan from Lentinus e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00B01D61/14B01D69/12B01D71/02B01D71/68
CPCB01D61/145B01D61/147B01D69/12B01D71/02B01D71/68C08B37/0003C08B37/0024
Inventor 秦令祥崔胜文高愿军
Owner 河南和生食品有限公司
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