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Application of indole-3-carboxaldehyde and its derivatives in control of plant diseases caused by phytopathogenic fungi

A technology for plant pathogenic fungi and plant diseases, applied in the direction of chemicals, applications, biocides, etc. for biological control, to achieve the effect of poor drug resistance, broad research and market application prospects, and high quality assurance

Active Publication Date: 2018-08-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report that indole-3-carboxaldehyde has an inhibitory effect on plant pathogenic fungi at home and abroad.

Method used

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  • Application of indole-3-carboxaldehyde and its derivatives in control of plant diseases caused by phytopathogenic fungi
  • Application of indole-3-carboxaldehyde and its derivatives in control of plant diseases caused by phytopathogenic fungi
  • Application of indole-3-carboxaldehyde and its derivatives in control of plant diseases caused by phytopathogenic fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Extraction of Pseudomonas ST4 metabolites and detection of its biological activity

[0065] 1. Extraction of strain ST4 metabolites

[0066] (1) Inoculate the overnight cultured ST4 bacterial solution on a PDA plate (13×13 cm), culture at 28°C for 48 hours, and wipe off the colonies with sterile gauze to obtain a fermentation medium;

[0067] (2) Cut the fermentation medium into small pieces and soak in 3 times the volume of a mixed solution composed of ethyl acetate:methanol:glacial acetic acid at a volume ratio of 80:15:5, shake for 2 hours, and filter to collect the supernatant; Use a rotary evaporator to remove organic solvents such as ethyl acetate and methanol in the supernatant, leaving the aqueous phase solution;

[0068] (3) Extract the aqueous phase solution 3 times with 3 times the volume of ethyl acetate, collect the aqueous phase and the organic phase respectively, evaporate the organic phase to dryness using a rotary evaporator, and concentrate ...

Embodiment 2

[0075] The separation and purification of the effective active substance of embodiment 2 Pseudomonas ST4

[0076] 1. Separation and purification method

[0077] The ethyl acetate extract obtained in Example 1 was sequentially separated by Claricep FlashSilica (CS) standard silica gel column chromatography, Butch medium pressure separator, high performance liquid chromatography and HPLC semi-preparative separation, the specific methods are as follows:

[0078] (1) Standard silica gel column chromatography separation:

[0079]1) Sample loading: Accurately weigh 5-6 g of the ethyl acetate extract of Pseudomonas ST4 each time, dissolve it with an appropriate amount of methanol, add twice the amount of 60-100 mesh silica gel, stir well, and air-dry the methanol. The silica gel containing the sample is added to the sample column, connected with the Claricep FlashSilica (CS) standard silica gel column, and connected with the Butch medium pressure separator and analytical grade metha...

Embodiment 3

[0098] Molecular formula and structure identification of embodiment 3 compound ST4-2-8

[0099] 1. Structural identification: ST4-2-8 obtained in Example 2 was analyzed by NMR and LC-MS to identify its chemical structure.

[0100] 2. Identification results:

[0101] The NMR analysis of ST4-2-8 shows that the compound contains 9 carbons, 7 Hs, 1 N and 1 O atom, and contains a parallel structure of a benzene ring and a pyrrole ring, at the 3rd position of benzopyrrole Containing an aldehyde group, the possible structure is indole-3-carbaldehyde; LC-MS analyzes the molecular weight of this substance to be 146.15, consistent with indole-3-carbaldehyde; Shown by HPLC analysis with finished product ( image 3 ), the retention time of ST4-2-8 and indole-3-carbaldehyde is 8.255min, and the peak shape and three-dimensional structure are completely consistent. Therefore, ST4-2-8 is identified as indole-3-carbaldehyde.

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Abstract

The invention discloses an application of indole-3-carboxaldehyde and its derivatives in the control of plant diseases caused by phytopathogenic fungi, and provides an application of the indole-3-carboxaldehyde or its derivative or pharmaceutically acceptable salts in the control of plant diseases caused by phytopathogenic fungi, or in the preparation of a pesticide for a biocontrol agent for controlling the plant diseases caused by phytopathogenic fungi. A result of antibacterial activity detection proves that the indole-3-carboxaldehyde and its derivatives have good inhibitory activity against the phytopathogenic fungi, have a good inhibitory effect on the growth and sexual cooperation of plant pathogenic fungi, and can block the formation of double-nuclear mycelia to prevent the phytopathogenic fungi from infecting plants, so the occurrence of plant fungal diseases is effectively inhibited, a reference is provided for the green control of the plant diseases caused by the phytopathogenic fungi, and the indole-3-carboxaldehyde and its derivatives have a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to the application of indole-3-carbaldehyde and its derivatives in the control of plant diseases caused by phytopathogenic fungi. Background technique [0002] Indole-3-carboxaldehyde is an important pharmaceutical intermediate, mainly used in the preparation of indole derivatives. Indole compounds are an important class of heterocyclic compounds with a wide range of biological activities. Indole secondary metabolites widely exist in cruciferous vegetables and a large number of marine organisms and actinomycetes. In recent years, its anticancer activity has attracted widespread attention. Currently, SU11248 (trade name: sunitinib), vinblastine (VLB, Vinblastine), vincristine (VCR, Vincristine), vindesine (VDS, Vindesine), vinorelbine (VBR, Vinorelbine), indirubin A small amount of varieties containing indole structures such as red have been put into us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/38A01P3/00
CPCA01N43/38
Inventor 张炼辉刘诗胤周佳暖贺飞林诺翘
Owner SOUTH CHINA AGRI UNIV
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