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Focusing method and focusing system

A focus method and focus search technology, applied in the focus method and system field of image photography, can solve the problems of weak fluorescent image signal, fluorescent substance attenuation, etc., and achieve the effect of fast speed and reduced quantity

Inactive Publication Date: 2018-07-31
GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD
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Problems solved by technology

Because the prior art focusing method for taking pictures of fluorescent images needs to irradiate laser light at a certain position in the sample area and take multiple photos, it will cause the fluorescent substances in this area to attenuate, which in turn leads to the fact that when the gene sequencing fluorescent images are officially taken, the Fluorescent image signal is weak

Method used

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  • Focusing method and focusing system
  • Focusing method and focusing system

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Embodiment Construction

[0042] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be described in detail below in conjunction with the accompanying drawings and implementation methods.

[0043] Please refer to figure 1 , the first embodiment of the present invention provides a focusing method, which includes steps S11-S15.

[0044] Searching and photographing step S11, using the mountain-climbing focus search method to adjust the focal length step by step to take multiple photographs of the target to obtain multiple first images.

[0045] In this embodiment, the target is a gene sequencing sample, and the gene sequencing sample includes multiple objects to be identified, and the objects are preferably objects with special structures or shapes. In a preferred embodiment, the target is preferably spherical or spherical, such as magnetic beads or microbeads. In this embodiment, by continuously adjusting the distance between th...

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Abstract

The invention relates to a focusing method. The method comprises a search and shooting step in which a hill climbing focusing search method is used to adjust a focal length in a stepping mode to carryout multiple times of shooting on a target to acquire multiple first images, a definition calculation step in which multiple definition values of the multiple first images are calculated, a shootingstop step in which shooting is stopped after an extreme value is judged to exist in the definition values of the multiple first images, a parameter acquisition step in which three focal lengths corresponding to three maximum definition values in the definition values of the multiple first images are acquired, and a focal length acquisition step in which the best focal length position for shootingthe target is calculated according to the three maximum definition values of the first images and the three focal lengths corresponding to the three maximum definition values. The invention also provides a focusing system. Compared with the prior art, the focusing method and the focusing system are quicker.

Description

technical field [0001] The present invention relates to the field of gene sequencing, and more specifically, the present invention relates to a focusing method and system for taking pictures of images. Background technique [0002] Genetic sequencers typically produce brightfield and fluorescence maps when photographing regions of a sequenced sample. Since fluorescence images need to use additional optical devices, such as filters, taking photos of fluorescence images needs to be refocused on the basis of the focal length of bright field images in order to take the clearest fluorescence images, which is essential for later gene sequencing Base recognition and quality scoring of the recognition process are very important. The focus adjustment method of the fluorescence image in the prior art is consistent with the automatic focus method of the bright field image, that is, change the focal length and take a set of pictures and calculate the definition of each picture, and sel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B7/38G03B13/36
CPCG02B7/38G03B13/36
Inventor 盛司潼冀高
Owner GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD
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