Super-resolution microimaging system and imaging method based on femtosecond pulse shaping

Abstract

The invention discloses a super-resolution microimaging system and an imaging method based on femtosecond pulse shaping. In the invention, a pulse shaping system is controlled by a computer control system so as to performing modulation in the aspects of phase position, intensity and polarization of light with different frequency components, so that femtosecond pulse laser becomes shaped pulse, ofwhich the intensity, polarization and frequency are related to time, as an exciting light; by using the exciting light for exciting a sample, particles, having different properties and types, in the sample have different response to the exciting light, so that responding intensity of a signal light relatively changes; when the pulse modulation changes, an signal light image is changed too; the computer control system, via a graphic processing algorithm, analyses and processes a plurality of the signal light images, thus acquiring a super-resolution image of the sample. The system and method overcome defects in methods in the prior art, has universality, is simple in optical path, allows wide-field observation, and is high in efficiency.

Description

technical field [0001] The invention relates to microscope technology, in particular to a super-resolution microscopic imaging system based on femtosecond pulse shaping and an imaging method thereof. Background technique [0002] Super-resolution microscopy has an important impact in the fields of living biological tissue imaging and material characterization. Among them, stimulated radiation depletion microscopy (STED), stochastic optical reconstruction microscopy (STORM), optical wave super-resolution imaging (SOFI), etc., utilize different characteristics of fluorescent molecules to obtain fluorescent molecules through modulation or acquisition. A microscopic technique for super-resolution imaging under different luminescence conditions. Compared with other imaging methods, the principle is relatively simple, it can perform super-resolution imaging of living tissue, and has the advantages of high spatial resolution. It has very huge applications in the fields of biomedic...

AI Technical Summary

Problems solved by technology

However, there is currently no method for multi-particle and multi-dimensional active regulation of particles.

For example, the STED and STORM methods belong to active regulation, but are based on single-particle luminescence, and do not realize multi-particle excitation control; the SOFI method belongs to multi-particle, but belongs to spontaneous luminescence, without multi-dimensionality, and active control; there are also some methods that use changing polarized light Exciting particles can excite multiple particles, but does not realize shaping pulse modulation, so that the complex energy level system of particles cannot be used, and its universality is limited

Images