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Feed silage agent containing Lactobacillus buchneri, preparation method and applications thereof

A technology of Lactobacillus brucei and silage agent, applied in the field of silage, can solve the problems of loss of nutrients, insufficient fermentation strength, and lack of inhibition strength of miscellaneous bacteria.

Active Publication Date: 2018-07-27
BEIJING HESWOF BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermentation of the silage in a short period of time is not strong enough, and it takes a long time for the silage speed to reach a pH value of 4, and the inhibition of miscellaneous bacteria is insufficient, which is likely to cause the loss of nutrients

Method used

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  • Feed silage agent containing Lactobacillus buchneri, preparation method and applications thereof
  • Feed silage agent containing Lactobacillus buchneri, preparation method and applications thereof
  • Feed silage agent containing Lactobacillus buchneri, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 active bacterium slime and protective agent

[0030] ⑴ Preparation of Lactobacillus Brucella HEW-A666 Active Sludge

[0031] Streak culture the strain HEW-A666 preserved in the glycerol tube on the MRS plate, 30°C, 48h, pick a single colony from the plate in 100mL seed medium, 30°C, 180r / min shaking culture for 24h, to obtain HEW-A666 Seed solution, activity up to 4.0×10 9 CFU / mL;

[0032] Take the live bacteria concentration as 10 9 Inoculate 1-5mL of seed solution in CFU / mL into 300mL medium, and carry out shake flask fermentation culture to obtain HEW-A666 primary seed solution;

[0033] Transfer the HEW-A666 primary seed liquid to the secondary seed medium of a 50L fermenter for secondary fermentation, and obtain the HEW-A666 secondary seed liquid after 5 hours of fermentation;

[0034] The HEW-A666 secondary seed liquid was transferred to the tertiary fermentation medium of a 5T fermenter for tertiary fermentation. After 24 hours...

Embodiment 2

[0057] Embodiment 2 A kind of preparation of feed silage containing lactobacillus brucella HEW--A666

[0058] The active bacterium slime of three kinds of bacterium described in embodiment 1 is mixed with protective agent in proportion, i.e. Lactobacillus Brucella active bacterium slime, Lactobacillus plantarum active bacterium slime, Bacillus coagulans active bacterium slime and protective agent are according to 0.75: The ratio (weight percentage) of 0.5:0.2:1.45 is mixed evenly.

[0059] After the active bacteria slime of the three kinds of bacteria and the protective agent are evenly mixed, pre-freeze at -80°C for 3-6 hours, and then freeze-dry for 30-48 hours to obtain a feed silage containing Lactobacillus Brucella. The feed silage contains The activity of Lactobacillus subtilis was 5.0×10 10 cfu / g, Lactobacillus plantarum activity is 3.0×10 10 cfu / g, the activity of Bacillus coagulans is 2.0×10 10 cfu / g.

Embodiment 3

[0060] Embodiment 3 A kind of preparation of feed silage containing Lactobacillus brucella HEW--A666

[0061] The difference from Example 2 is that the active sludge of Lactobacillus Brucella, the active sludge of Lactobacillus plantarum, the active sludge of Bacillus coagulans and the protective agent are uniformly mixed according to the ratio (percentage by weight) of 0.5:0.2:0.1:1.25.

[0062] After the active bacteria slime of the three kinds of bacteria and the protective agent are evenly mixed, pre-freeze at -80°C for 3-6 hours, and then freeze-dry for 30-48 hours to obtain a feed silage containing Lactobacillus Brucella. The feed silage contains The activity of Lactobacillus subtilis was 3.5×10 10 cfu / g, Lactobacillus plantarum activity is 1.8×10 10 cfu / g, the activity of Bacillus coagulans is 5.0×10 9 cfu / g.

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Abstract

The present invention provides a feed silage agent containing Lactobacillus buchneri, wherein the silage agent comprises Lactobacillus buchneri, Lactobacillus plantarum and Bacillus coagulans, the Lactobacillus buchneri is preserved in the China General Microbiological Culture Collection Center on August 9, 2017, has the preservation number of CGMCC NO.14508, and has the classification name of Lactobacillus buchneri, the Lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center on May 27, 2016, has the preservation number of CGMCC NO.12554, and has theclassification name of Lactobacillus plantarum, and the Bacillus coagulans is preserved in the China General Microbiological Culture Collection Center on on May 27, 2016, has the preservation numberof CGMCC No.12553, and has the classification name of Bacillus coagulans. According to the present invention, with the application of the feed silage agent containing Lactobacillus buchneri in the whole-plant corn silage, the pH value of the corn silage can be significantly reduced, the contents of acetic acid and lactic acid can be obviously increased, and the content of lactic acid bacteria canbe obviously improved.

Description

technical field [0001] The invention belongs to the field of silage feed, and particularly relates to a feed silage agent containing Lactobacillus brucei and a preparation method and application thereof. Background technique [0002] Silage is a succulent feed prepared and preserved by the fermentation of microorganisms such as lactic acid bacteria under anaerobic conditions after proper processing of fresh green feed. It can not only maintain the green and juicy characteristics of raw materials, but also It has a special sour aroma, soft texture, rich nutrition and good palatability. In recent years, with the rapid development of the animal husbandry economy, the use of silage to provide a balanced supply of green and juicy feed throughout the year has become an important means for the healthy development of the dairy industry. In order to obtain the ideal fermentation quality, the number of lactic acid bacteria in the silage should reach 10 5 cfu / g or above. However, in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18C12R1/225C12R1/25C12R1/07
CPCA23K30/18C12N1/20A23V2400/11A23V2400/169
Inventor 李雪平刘建芳尹望杜志琳刘爱君
Owner BEIJING HESWOF BIOTECH CO LTD
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