Long-time storage method of lactobacillus-plantarum liquid preparation

Abstract

The invention discloses a long-time storage method of a lactobacillus-plantarum liquid preparation. The long-time storage method comprises the following steps of (1) carrying out purification culturetreatment on lactobacillus plantarum, so as to obtain a lactobacillus-plantarum liquid bacterial solution; (2) adding a solid MRS formula substance into the lactobacillus-plantarum liquid bacterial solution; (3) storing the lactobacillus-plantarum liquid bacterial solution into which the solid MRS formula substance is added in a low-temperature or room-temperature condition. According to the long-time storage method, a solid MRS component is added into the lactobacillus-plantarum liquid bacterial solution and under appropriate temperature condition (a low temperature or a normal temperature),the storage time of the lactobacillus-plantarum liquid preparation can be effectively prolonged; the certain viable count of the lactobacillus-plantarum liquid preparation is ensured, and the storagemethod provided by the invention is simple and reasonable, is quick to operate and has a favorable application prospect.

Description

technical field [0001] The invention relates to a long-term storage method for a liquid preparation of Lactobacillus plantarum. Background technique [0002] Lactobacillus plantarum is a kind of lactic acid bacteria. The optimum growth temperature is 30℃~35℃. It is anaerobic or facultative anaerobic. The optimum pH is about 6.5. It belongs to homofermentative lactic acid bacteria. Lactobacillus plantarum is closely related to human life. It is a lactic acid bacteria commonly found in butter, meat and many vegetable fermented products. It can colonize the intestinal tract and play a beneficial role. [0003] Lactobacillus plantarum has an important influence on the intestinal microbial flora, and has strong application potential in food fermentation, industrial lactic acid fermentation, and medical care. In recent years, as food, feed and other products that need large-scale production have gradually increased the requirements for fermentation technology, it is more importan...

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Problems solved by technology

However, these studies did not give a comparison of the number of viable bacteria after preservation and before preservation, and the duration of the research observation was not long enough, and there were certain difficulties in actual operation, so there was no practical application

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