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Method for promoting persea americana stem to induce callus tissues and sterile buds

A technology of callus and avocado, which is applied in the field of promoting the induction of callus and sterile buds of avocado stems, which can solve the problems of easy necrosis of cultured tissues, low dedifferentiation ability, and reduced pollution rate, and achieve easy disinfection , not easy to necrosis, the effect of controlling the pollution rate

Inactive Publication Date: 2018-07-24
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the defects of low dedifferentiation ability, difficult disinfection and easy necrosis of culture tissue in the prior art, the present invention provides a method for promoting the induction of callus and sterile buds of avocado stems; The method is easy to operate, can significantly increase the induction rate of callus and regenerated shoots, and significantly reduce the pollution rate, and can obtain better quality callus and sterile shoots through this method, and realize the isolation of adult stems of avocado The role of efficient and rapid propagation

Method used

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  • Method for promoting persea americana stem to induce callus tissues and sterile buds
  • Method for promoting persea americana stem to induce callus tissues and sterile buds
  • Method for promoting persea americana stem to induce callus tissues and sterile buds

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 adopts the following steps to compare the situation of obtaining avocado plant stem segment induced callus and aseptic buds with two different explants of semi-childhood stem segment and adult state stem segment:

[0024] (1) Acquisition of different explants

[0025] ① Acquisition of stem segments at the half-childhood stage: scions were selected from the adult avocado plants of 'Dusa' and 'Duke7' respectively, and grafted to the seedlings. New branches can be re-emerged in the next year. The middle and upper parts of these re-emerged branches are Semi-lignified branches with axillary buds, that is, semi-childhood branches, which have a certain degree of juvenile characteristics; select this kind of semi-childhood branches, and use sterilized scissors to cut off healthy long stems without pests and diseases. The length of the stems is 20cm. 0.5-1cm in diameter;

[0026] ②Acquisition of adult stem segments: Select semi-lignified branches with axillary buds...

Embodiment 2

[0036] Embodiment 2 adopts the following steps to compare the impact of different disinfection methods on the induction of callus and sterile buds of avocado stem explants:

[0037] (1) Acquisition of explants: scions were selected from adult avocado plants of 'Dusa' and grafted onto seedlings. New shoots could be sprouted in the next year, and the upper part of these sprouted branches were semi-woody with axillary buds. The branches, that is, the branches of the semi-childhood, they have a certain degree of juvenile characteristics. Select this kind of semi-childhood branches, and use sterilized scissors to cut off healthy long stems without diseases and insect pests. The stems are 20cm long and 0.5-1cm in diameter;

[0038] (2) Explant pretreatment: quickly bring the selected long stem section back indoors, remove the leaves, cut into 5-8cm long sections with sterile scissors, place them in a clean container, and rinse them under running water for 30 minutes;

[0039] (3) Disi...

Embodiment 3

[0050] Embodiment 3 adopts the following steps to compare the situation of avocado stem segment explants inducing callus and aseptic buds under different culture methods:

[0051] (1) Acquisition of explants: Select scions from adult avocado plants of 'Dusa' and graft them to seedlings. New branches can be re-emerged in the next year. These re-emerged branches are semi-woody with axillary buds in the upper part. The branches, that is, the branches of the semi-childhood, they have a certain degree of juvenile characteristics. Select this kind of semi-childhood branches, and use sterilized scissors to cut off healthy long stems without diseases and insect pests. The stems are 20cm long and 0.5-1cm in diameter;

[0052] (2) Explant pretreatment: quickly bring the selected long stem section back indoors, remove the leaves, cut into 5-8cm long sections with sterile scissors, place them in a clean container, and rinse them under running water for 30 minutes;

[0053] (3) Disinfectio...

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Abstract

The invention discloses a method for promoting persea americana stem to induce callus tissues and sterile buds. The method comprises the following steps: (1) selecting explants; (2) pretreating the explants; (3) sterilizing; (4) inoculating; (5) culturing; (6) obtaining the callus tissues and regeneration buds. The method disclosed by the invention has the following beneficial effects that (1) tissue culture is carried out by utilizing stems of persea americana during a semi-juvenile phase as the explants; compared with adult stems, a germinating rate of the regeneration buds of the stems ishigher, and tissues are not easy to be necrosis; (2) compared with the adult stems, the stems in the semi-juvenile phase are easy to sterilize, and the purposes of not affecting an induction effect and not damaging a tissue structure of the explants can be achieved by strictly controlling the time and the concentration of a sterilizing agent; the contamination rate can be controlled to be 15 percent or less through the method; (3) through dark culture, the callus tissues can be induced by seven days, the regeneration buds can be induced by 15 days, the activity of the callus tissues after 15-day illumination culture can be higher, and the regeneration buds can grow more quickly.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for promoting the induction of callus and sterile buds of avocado stem segments. Background technique [0002] Avocado (Persea americana Mill.) belongs to Lauraceae (Lauraceae). Avocado is a perennial evergreen tree fruit tree, also known as avocado. It is native to Central and South America and is an ideal high-energy and low-sugar fruit. my country began large-scale introduction and trial planting in the 1980s, mainly concentrated in Guangxi, Hainan and other places. In recent years, with the improvement of people's quality of life, the development of avocado in my country's hot areas has significant economic benefits. [0003] At present, the lack and breeding of good rootstocks in production have greatly limited the development of avocado planting and industry in China. In recent years, the South Subtropical Crops Research Institute of the Chinese Ac...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 许文天王松标高玉尧武红霞罗纯梁清志姚全胜
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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