Device and method for detecting cell size and electrical parameter of unicell irrelevant to size at high throughput

Abstract

The disclosure of the invention provides a device and a method for detecting the cell size and the electrical parameter of a unicell irrelevant to size at the high throughput. The device comprises a micro-fluidic chip comprising a main compression channel, an auxiliary compression channel, electrodes, an impedance measurement module and a data processing module; the cross sectional area of the main compression channel is less than the cross sectional area of the cell, so that the cell stretches and flows along the main compression channel; the auxiliary compression channel and the main compression channel are crossed and communicated, the cross sectional area of the auxiliary compression channel is less than the cross sectional area of the side edge of the stretched cell; the electrodes are respectively arranged at the two ends of the auxiliary compression channel, and an electric conduction channel is formed between the two electrodes; the impedance measurement module is connected with the wo electrodes and measures the impedance data of the two electrodes when the cell flows by the cross position of the main compression channel and the auxiliary compression channel; the data processing module is connected with the impedance measurement module, and the cell size and the electrical parameter of the unicell irrelevant to size are calculated according to the acquired impedance data. The method for detecting by using the device is further provided. The disclosure achieves the high-throughput detection of the above parameter, and the peak detection throughput is up to 100 cellsper second.

Description

technical field [0001] The present disclosure relates to the field of microfluidic technology, in particular to a device and method for high-throughput detection of cell size and size-independent electrical parameters of single cells. Background technique [0002] Cell heterogeneity is a biological phenomenon ubiquitous from bacterial cells to eukaryotic cells. Studying the heterogeneity of single cells is of great significance for understanding the differentiation process of stem cells and tumor diagnosis and treatment. Among the many characterization methods used to study single-cell heterogeneity, cell size is an important physical parameter. In addition, single-cell electrical properties, as an important single-cell biophysical property, have been proven to be used to distinguish different tumor cells and blood cells. Single-cell electrical properties include membrane capacitance and cytoplasmic electrical resistance. Considering that cells vary in size, it is more mea...

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Problems solved by technology

However, there is a solution between the electrodes, which will form a large leakage current, and it is impossible to obtain size-independent single-cell electrical parameters.

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