Device and method for high-throughput detection of cell size and size-independent single-cell electrical parameters

A detection device and high-throughput technology, applied in the field of microfluidics, can solve the problems of inability to obtain single-cell electrical parameters, large leakage current, etc., and achieve the effects of convenient parallel and series arrangement, efficient measurement, and avoiding data processing.

Active Publication Date: 2022-07-19
INST OF ELECTRONICS CHINESE ACAD OF SCI +1
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Problems solved by technology

However, there is a solution between the electrodes, which will form a large leakage current, and it is impossible to obtain size-independent single-cell electrical parameters.

Method used

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  • Device and method for high-throughput detection of cell size and size-independent single-cell electrical parameters
  • Device and method for high-throughput detection of cell size and size-independent single-cell electrical parameters
  • Device and method for high-throughput detection of cell size and size-independent single-cell electrical parameters

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Embodiment Construction

[0063] The present disclosure provides a device and method for high-throughput detection of cell size and size-independent single-cell electrical parameters, wherein the size-independent single-cell electrical parameters include cell membrane specific capacitance and cytoplasmic conductivity. When cells flow through cross-connected compression channels, Impedance data at the intersection position, calculate cell size, cell membrane specific capacitance and cytoplasmic conductivity, the entire detection process is not limited to high-speed optical photography, and does not require complex image processing. detection.

[0064] In order to make the objectives, technical solutions and advantages of the present disclosure more clear, the present disclosure will be further described in detail below with reference to the specific embodiments and the accompanying drawings.

[0065] figure 1 This is a schematic diagram of a high-throughput detection device for cell size and size-indep...

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Abstract

The present disclosure provides a device and method for high-throughput detection of cell size and size-independent single-cell electrical parameters. The device includes: a microfluidic chip, including: a main compression channel, the cross-sectional area of ​​which is smaller than the cell cross-sectional area, so that the cell Tension and flow are generated along it; the auxiliary compression channel crosses and communicates with the main compression channel, and its cross-sectional area is smaller than the lateral cross-sectional area of ​​the stretched cell; and electrodes are respectively arranged at both ends of the auxiliary compression channel, between the two electrodes A conductive channel is formed between the two electrodes; the impedance measurement module is connected to the two electrodes, and the impedance data between the two electrodes is measured when the cells flow through the intersection of the main compression channel and the auxiliary compression channel; and the data processing module is connected to the impedance measurement module, according to the obtained impedance data Data calculation of cell size, size-independent single-cell electrical parameters. Methods of detection using the device are further provided. The present disclosure realizes high-throughput detection of the aforementioned parameters, and the peak detection throughput can reach 100 cells / s.

Description

technical field [0001] The present disclosure relates to the technical field of microfluidics, and in particular, to a device and method for high-throughput detection of cell size and size-independent electrical parameters of single cells. Background technique [0002] Cellular heterogeneity (heterogeneity) is a ubiquitous biological phenomenon from bacterial cells to eukaryotic cells. Studying the heterogeneity of single cells is of great significance for understanding the process of stem cell differentiation and tumor diagnosis and treatment. Among the many characterization methods used to study single-cell heterogeneity, cell size is an important physical parameter. In addition, single-cell electrical properties, as an important single-cell biophysical property, have been demonstrated to distinguish different tumor cells and blood cells. Single-cell electrical properties include cell membrane capacitance and cytoplasmic resistance, and considering cell size differences,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/10G01N15/00
CPCG01N15/00G01N15/10G01N15/1031G01N15/1056G01N2015/1006G01N2015/1087G01N2015/0065
Inventor 陈健张毅赵阳王棵陈德勇王军波黄成军
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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