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Ganoderma-lucidum-intercropping cultivation method for litchi chinensis bacteria

A cultivation method and lychee technology, which are applied in the field of artificial cultivation of wild fungi, can solve the problems of unstable symbiotic relationship and affect cultivation stability, and achieve the effects of stable cultivation conditions, increased protein content, and increased polysaccharide content.

Inactive Publication Date: 2018-07-20
FOSHAN SANSHUI JIAXIN AGRI TECH RES INST GENERAL PARTNERSHIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This invention prevents termites from migrating by spraying the crude extract of litchi wood to maintain the symbiotic relationship between litchi fungus and termites, and realize the artificial cultivation of litchi fungus. However, affected by other cultivation and environmental factors, the symbiotic relationship is easily unstable, which in turn affects cultivation. stability

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0022] A method for cultivating litchi fungus intercropped with Ganoderma lucidum, said method comprising the following steps:

[0023] (1) Preparation of culture medium: Stir the raw materials used in the culture medium evenly, control the water content to be 60%, bag and sterilize;

[0024] (2) Inoculate the cultivars of ganoderma: put the fungus bags obtained in step (1) into a sterile room to inoculate the cultivars of ganoderma after cooling down, then move the fungus bags into the cultivation room, and overturn the fungus bags every 3-6 days;

[0025] (3) Inoculation of litchi fungus cultivars: after step (2) bacteria bags were cultivated for 7 days, the bacteria bags were moved into the aseptic room for repacking, inoculated with litchi fungus cultivars, and continued to be moved into the cultivation room;

[0026] (4) Germination stage: keep the temperature in the cultivation room at 20°C, the relative air humidity at 60%, and ventilate for 0.5h. When the ganoderma myc...

Embodiment 2

[0031] A method for cultivating litchi fungus intercropped with Ganoderma lucidum, said method comprising the following steps:

[0032] (1) Preparation of culture medium: Stir the raw materials used in the culture medium evenly, control the water content to be 68%, bag and sterilize;

[0033] (2) Inoculate the cultivars of ganoderma: put the fungus bags obtained in step (1) into a sterile room to inoculate the cultivars of ganoderma after cooling down, then move the fungus bags into the cultivation room, and overturn the fungus bags every 4.5 days;

[0034] (3) Inoculation of litchi fungus cultivars: after step (2) bacteria bags were cultivated for 9 days, the bacteria bags were moved into the aseptic room for repacking, inoculated with litchi fungus cultivars, and continued to be moved into the cultivation room;

[0035] (4) Germination stage: keep the temperature in the cultivation room at 24°C, the relative humidity of the air is 70%, and ventilate for 1 hour. When the gano...

Embodiment 3

[0039] A method for cultivating litchi fungus intercropped with Ganoderma lucidum, said method comprising the following steps:

[0040] (1) Preparation of culture medium: Stir the raw materials used in the culture medium evenly, control the water content to be 75%, bag and sterilize;

[0041] (2) Inoculation of ganoderma cultivars: put the fungus bags obtained in step (1) into a sterile room to inoculate ganoderma cultivars after cooling down, then move the fungus bags into the cultivation room, and overturn the fungus bags every 6 days;

[0042] (3) Inoculation of litchi fungus cultivars: after step (2) bacteria bags were cultivated for 12 days, the bacteria bags were moved into the aseptic room for repacking, inoculated with litchi fungus cultivars, and continued to be moved into the cultivation room;

[0043] (4) Germination stage: Keep the temperature in the cultivation room at 28°C, the relative air humidity at 80%, and ventilate for 1.5 hours. When the mycelium of Ganode...

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Abstract

The invention discloses a ganoderma-lucidum-intercropping cultivation method for litchi chinensis bacteria. The method comprises the following steps: (1) culture medium preparing; (2) ganoderma lucidum cultivated species inoculation: placing fungus bags into a sterile room after the fungus bags obtained in the step (1) are cooled, inoculating the fungus bags with a ganoderma lucidum cultivated species, then, shifting the fungus bags in a culture room, and overturning the fungus bags every 3 to 6 days; (3) litchi chinensis bacterium cultivated species inoculation: shifting the fungus bags in asterile room for subpackaging after the fungus bags in the step (2) are cultured for 7 to 12 days, inoculating the fungus bags with a litchi chinensis bacterium cultivated species, and continuing to shift the fungus bags in a culture room; (4) spawn running stage; and (5) ripening stage. Compared with the prior art, the method has the beneficial effects that a symbiotic relationship between the litchi chinensis bacteria and ganoderma lucidum is established, and the dependency on termites of the litchi chinensis bacteria is broken through; when the inoculum size of the ganoderma lucidum is 10%,the content of protein in litchi chinensis bacterium fruiting bodies can be increased; when the inoculum size of the litchi chinensis bacteria is 3%, the content of polysaccharides in the ganoderma lucidum can be increased; and cultivation conditions are stable, and the popularization of the litchi chinensis bacteria is facilitated.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of wild fungi, and in particular relates to a method for cultivating litchi fungi intercropped with ganoderma lucidum. Background technique [0002] Litchi chinensis Bacterium (Litchi chinensis Bacterium) is an edible fungus that grows rapidly on moist termite nests in litchi forests in Guangdong. It is known as the "king of bacteria". Neng Guanyu is so named because the lychee fungus has unique flavors such as tender meat, fragrant taste, and fresh and sweet taste. In addition, it has very strict requirements on the growth environment. It only grows on the moist termite nests in the lychee forest, and the annual growth cycle is only short. As short as 20 days, it is very precious, but there are very few studies on its artificial cultivation. [0003] Application No. 201611159283.X discloses a method for artificially cultivating litchi bacteria, which is characterized in that the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCC05D3/02C05G1/00C05D9/02C05F11/00C05F11/02
Inventor 曹丽萍
Owner FOSHAN SANSHUI JIAXIN AGRI TECH RES INST GENERAL PARTNERSHIP
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