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Detection kit of super gonorrhea strain and detection method thereof

A detection kit and a technology for gonorrhea bacteria, which are applied in the field of medical detection, can solve the problem of no accurate detection of super gonorrhea strains, etc., and achieve the effects of convenient and fast detection, cost reduction, controllability and accuracy.

Inactive Publication Date: 2018-07-17
李梅秀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no effective and accurate detection method for super gonorrhea strains

Method used

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  • Detection kit of super gonorrhea strain and detection method thereof
  • Detection kit of super gonorrhea strain and detection method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Human peripheral blood (PBMC) samples were derived from super gonorrhea infection patients and non-super gonorrhea infection populations, and the number was 127 samples to be tested.

[0041] 10 mL of blood was collected from each individual with heparin sodium collection tubes, and transported to the laboratory within 6 hours at room temperature.

[0042] The detection steps of each sample to be tested are as follows:

[0043] (1) Separate the PBMC with the separation medium, resuspend the separated cells in serum-free medium, and the cell concentration is 1.5×10 5Cells / mL; Add the resuspended cells into a PVDF96-well plate, add 100 μL to each reaction well, add each sample to 10 wells, and make 10 repetitions;

[0044] (2) Add 50 μL of a mixture consisting of serum-free medium and 6 polypeptides with a final concentration of 5 μM to each reaction well, mix well and carry out co-incubation; set a positive control and a negative control, and add high Specific mouse an...

Embodiment 2

[0053] Human peripheral blood (PBMC) samples were derived from super gonorrhea infection patients and non-super gonorrhea infection populations, and the number was 127 samples to be tested.

[0054] 10 mL of blood was collected from each individual with heparin sodium collection tubes, and transported to the laboratory within 6 hours at room temperature.

[0055] The detection steps of each sample to be tested are as follows:

[0056] (1) Separate the PBMC with the separation medium, resuspend the separated cells in serum-free medium, and the cell concentration is 3.5×10 5 Cells / mL; Add the resuspended cells into a PVDF96-well plate, add 100 μL to each reaction well, add each sample to 10 wells, and make 10 repetitions;

[0057] (2) Add 50 μL of a mixture consisting of serum-free medium and 6 polypeptides with a final concentration of 5 μM to each reaction well, mix well and carry out co-incubation; set a positive control and a negative control, and add high Specific mouse a...

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Abstract

The invention provides a detection kit of a super gonorrhea strain and a detection method thereof. The kit comprises six polypeptides represented by the amino acid sequence SEQ ID NO.1-SEQ ID NO.6. The detection method includes the steps that 1, a sample to be tested is separated through a separating medium, and the separated cells are resuspended in a serum-free medium; the six polypeptides represented by the amino acid sequence SEQ ID NO.1-SEQ ID NO.6 are added to the resuspended cells for co-incubation, and a high-specificity mouse anti-human IgG antibody is added to a co-incubation systemfor an antigen antibody reaction; 3, washing is conducted on the system after the antigen antibody reaction, a chromogenic substrate is then added for a chromogenic reaction, and the number of spots generated by the reaction is counted. The kit has the advantages of achieving artificial complete sequence synthesis, having convenient production, being high in stability and specificity and simple inoperation, and greatly reducing the cost.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a detection kit for super gonorrhea strains and a detection method thereof. Background technique [0002] Super gonorrhea strain disease, also known as "H041", is a new type of gonorrhoeae caused by gene mutation or plasmid exchange caused by human-dog crossbreeding. The bacteria are resistant to all currently used antibiotics. It was first discovered in Japan in 2011, and infections have also appeared in places such as the United States and Norway. In 2013, the U.S. Department of Health warned that the bacteria could be more contagious than AIDS. The H041 virus is quite aggressive and can infect more people in a short period of time. The infected person may develop symptoms of septic shock or even die within a few days. [0003] Super gonorrhea strains can be specifically combined with antigens and antibodies. According to the number of antibodies to supe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/571
CPCG01N33/68G01N33/571
Inventor 李梅秀
Owner 李梅秀
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