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Acanthopanax sessiliflorus micromolecule concentrated powder and preparation method and application of acanthopanax sessiliflorus micromolecule concentrated powder

A short stem five plus, small molecule technology, applied in the field of preparation, short stem five plus small molecule concentrated powder, can solve the problems of high average molecular weight, low polypeptide purity, poor solubility, etc., and achieve low molecular weight, good solubility, and transformation high rate effect

Inactive Publication Date: 2018-07-13
丹东五加生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned Duangengwujia small-molecule concentrated powder has the advantages of low molecular weight, high content of small-molecule polypeptides and good solubility, which effectively alleviates the problems of the existing Duangengwujia polypeptide preparations, which have low purity, poor solubility, and average molecular weight. big problem

Method used

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  • Acanthopanax sessiliflorus micromolecule concentrated powder and preparation method and application of acanthopanax sessiliflorus micromolecule concentrated powder
  • Acanthopanax sessiliflorus micromolecule concentrated powder and preparation method and application of acanthopanax sessiliflorus micromolecule concentrated powder

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preparation example Construction

[0044] According to one aspect of the present invention, a kind of preparation method of short stem five plus small molecule concentrated powder, described method comprises the following steps:

[0045] (a) Decoction preparation: Thresh and dry the fresh fruit of Duangengwujia and crush it into a fine powder with a mesh size of 80-100, then add water to heat up to 90-95°C and decoct for 10-20 minutes to obtain a decoction;

[0046] (b) stage enzymolysis: the decoction liquid is divided into three stages and sequentially used alkaline protease, neutral protease and acid protease to carry out enzymolysis three times under the respective optimum pH value environment, and the enzymolysis solution is obtained;

[0047] (c) Enzyme inactivation: heat up the enzymatic hydrolysis solution to 90-95°C, inactivate the enzyme for 30-60 minutes to inactivate the protease;

[0048] (d) Concentration and drying: filter and remove residue from the enzymatic hydrolysis solution after deactivati...

Embodiment 1

[0071] A preparation method of short stem five plus small molecule concentrated powder, said method comprising the following steps:

[0072] (a) Decoction preparation: thresh and dry the fresh fruit of Wujia Acanthopanax, crush it into a fine powder over 80 meshes, then add water and heat it up to 90°C and decoct for 10 minutes to obtain a decoction solution.

[0073] In the step (a), the mass ratio of the short stem five plus fine powder to the water added is 1:20;

[0074] (b) Phase enzymolysis: first, cool the decoction to 50°C, add alkaline protease with an activity unit of 500,000 in 1% of the weight of the decoction, adjust the pH to 8, keep at 50°C, and perform enzymolysis for 2 hours;

[0075] Subsequently, the temperature was lowered to 40°C, and neutral protease with an activity unit of 150,000 of 1 to 3% of the weight of the decoction liquid was added, the pH was adjusted to 6, and the enzymolysis was carried out at 40°C for 2 hours;

[0076] Finally, heat up to 50...

Embodiment 2

[0080] A preparation method of short stem five plus small molecule concentrated powder, said method comprising the following steps:

[0081] (a) Decoction preparation: thresh and dry the fresh fruit of Duangengwujia and crush it into a fine powder over 100 meshes, then add water and heat it up to 95°C and decoct for 20 minutes to obtain a decoction solution.

[0082] In the step (a), the mass ratio of the short stem five plus fine powder to the water added is 1:40;

[0083] (b) Phase enzymolysis: first, cool the decoction to 60°C, add alkaline protease with an activity unit of 500,000 in 3% of the weight of the decoction, adjust the pH to 9, keep at 60°C, and perform enzymolysis for 3 hours;

[0084] Subsequently, the temperature was lowered to 50°C, and neutral protease with an activity unit of 150,000 of 1 to 3% of the weight of the decoction liquid was added, the pH was adjusted to 7, and the enzymolysis was carried out at 50°C for 3 hours;

[0085] Finally, heat up to 60°...

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Abstract

The invention provides an acanthopanax sessiliflorus micromolecule concentrated powder and a preparation method and application of the acanthopanax sessiliflorus micromolecule concentrated powder, andrelates to the technical field of extraction of natural plants. The acanthopanax sessiliflorus micromolecule concentrated powder adopts the phased enzymolysis cutting technique, alkaline protease, neutral protease and acid proteinase are respectively used for enzymolysis for three times at the respectively mostly appropriate values, the reaction modification is conducted while the enzymolysis cutting is implemented, the unexpected technical effect is achieved, the prepared acanthopanax sessiliflorus concentrated powder has the advantages of small molecular weight, good solubleness, and high conversion rate of proteins into micro-molecule polypeptides, and the problems of low polypeptide purity, poor solubleness and large average molecular weight of the existing micro-molecule polypeptidepolypeptide preparation are effectively alleviated. The acanthopanax sessiliflorus micromolecule concentrated powder can be widely applied to preparing medicines, healthcare products, beverages and functional nutritious foods.

Description

technical field [0001] The invention relates to the technical field of natural plant extraction, in particular to a short stem five plus small molecule concentrated powder, a preparation method and an application thereof. Background technique [0002] Short-stemmed Acanthopanax, also known as Wujia Ginseng, is a medicinal and edible shrub of Araliaceae. Li Shizhen, a famous medical scientist in the Ming Dynasty, has already discussed in "Compendium of Materia Medica" that "it is better to have a handful of Wujia than a car full of gold and jade". Duangengwujia is rich in trace elements such as selenium, iron, calcium, zinc, etc. Traditional Chinese medicine believes that Duangengwujia has the effects of dispelling wind and dampness, promoting blood circulation and removing blood stasis, strengthening the stomach and diuresis. [0003] With the development of society and economy, people pay more and more attention to the deep processing of short stem five plus. Recent studie...

Claims

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Application Information

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IPC IPC(8): A61K36/254A61K9/14C12P21/06A23L33/105A23L33/18A23L2/39
CPCA23L2/39A23L33/105A23L33/18A23V2002/00A61K9/14A61K36/254A61K2236/15A61K2236/19A61K2236/331A61K2236/51C12P21/06A23V2200/30A23V2250/21A23V2250/55
Inventor 张春艳李峰孙宝俊郭军田甜
Owner 丹东五加生物科技有限公司
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