Preparation method of atherosclerotic mouse model with NOD (Non-Obese Diabetic) genetic background

Abstract

The invention relates to a preparation method of an atherosclerotic mouse model with NOD (Non-Obese Diabetic) genetic background, and belongs to the technical fields of genetic engineering and geneticmodification. For the first time, ApoE (Apolipoprotein E) and LDLR (Low-Density Lipoprotein Receptor) genes in a NOD genetic background mouse are knocked out at the same time to obtain a gene knockout mouse model, which can induce atherosclerotic atherosclerosis, of the NOD genetic background mouse. The aorta of the atherosclerotic mouse model with the NOD genetic background has obvious atherosclerotic plaque formation. Compared with the traditional classic atherosclerotic model C57B / L6 background ApoE gene knockout mouse, the atherosclerotic mouse model has consistent incidence degree. By adopting the method disclosed by the invention, a brand new atherosclerosis gene knockout mouse model with the NOD genetic background is provided for researchers, and is of great value for the basic theoretical research as well as clinical diagnosis and treatment of atherosclerosis.

Description

technical field [0001] The invention relates to a method for preparing an atherosclerosis mouse model with NOD genetic background, belonging to the technical field of genetic engineering and genetic modification. Background technique [0002] Atherosclerosis is one of the main causes of cardiovascular and cerebrovascular diseases. In-depth research on its mechanism will help to solve the difficult problem of curing and high mortality of cardiovascular and cerebrovascular diseases. NOD mice are a non-obese diabetic strain, and the cumulative incidence of diabetes at the age of 30 weeks is 60-80% for males and 20-30% for males, respectively. Female mice have insulin-dependent diabetes, and the clinical symptoms are quite similar to human type I diabetes. Mice are the most widely used mouse strains in the research of type 1 diabetes and the establishment of humanized models, but gene knockout models of NOD genetic background mice are still extremely scarce, especially in the fi...

AI Technical Summary

Problems solved by technology

NOD mice are a non-obese diabetic strain, and the cumulative incidence of diabetes at the age of 30 weeks is 60-80% for males and 20-30% for males, respectively. Female mice have insulin-dependent diabetes, and the clinical symptoms are quite similar to human type I diabetes. Mice are the most widely used mouse strains in the research of type 1 diabetes and the establishment of humanized models, but gene knockout models of NOD genetic background mice are still extremely scarce, especially in the field of atherosclerosis research

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