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Zinc-rich bacterial strain and application thereof

A technology of bacteria strains and bacteria powder, applied in zinc-rich bacteria strains and its application fields, can solve problems such as limiting market development space, and achieve the effect of promoting various digestive enzyme activities in the intestinal tract, simple operation process, simple and safe process

Active Publication Date: 2018-06-29
山东德信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of product with a single characteristic of the application strain greatly limits the development space of the market

Method used

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  • Zinc-rich bacterial strain and application thereof
  • Zinc-rich bacterial strain and application thereof
  • Zinc-rich bacterial strain and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0035] 6) Preparation of Bacillus subtilis zinc: Cultivate the domesticated Bacillus subtilis under the final optimized medium and culture conditions, after culturing for a certain period of time, centrifugation, washing to obtain the precipitate, and drying at 55°C to obtain the Bacillus subtilis zinc.

[0036] The preparation parameters of Bacillus subtilis zinc are: ①Medium formula: peptone 10g / L, yeast powder 5g / L, NaCl10g / L, glucose 4.50g / L, zinc concentration 33.9mg / L, pH 6.57; ②Culturing conditions: The inoculum amount is 3%, the culture temperature is 29.50℃, the culture time is 48h, and the rotation speed is 180r / min.

[0037] When the added amount of zinc is 33.9 mg / kg, the final dry weight of Bacillus subtilis zinc is 1.44193 g / L and the amount of organic zinc is 2511.55 mg / kg.

[0038] The method of the present invention will be described in detail below in conjunction with specific implementation cases.

Embodiment 1

[0039] Example 1: Preparation of Bacillus subtilis zinc

[0040] 1) Domestication of strains: the Bacillus subtilis powder that is commonly used in feed supplements is activated, separated and purified to obtain Bacillus subtilis strains; then the characteristics of microorganisms that can gradually adapt to unfavorable growth environment factors are used to gradually improve Acclimation in the medium of zinc sulfate concentration;

[0041] 2) Identification and physicochemical properties of strains: Identify the strains of Bacillus subtilis after preliminary domestication, and compare the morphology, enzyme activity, small peptides, amino acids, etc. with the strains before domestication to further determine the domesticated subtilis Bacillus is the target species.

[0042] The final screening obtained Bacillus subtilis (Bacillus subtilis) NZ56 strain, which was deposited on January 31, 2018 at No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, China Microbial Culture Col...

Embodiment 2

[0054] Example 2 Establishment of fermentation method

[0055] 1. The steps of the present invention are as follows:

[0056] 1) Determination of the balance point (measurement index): According to the growth characteristics of the strain and the tolerance to metal zinc ions, the bacterial content and the amount of organic zinc in the bacterial body are determined as the final measurement index.

[0057] 2) Optimization of the culture medium and culture conditions: The content of glucose, pH, culture temperature, rotation speed, inoculation amount, etc. are optimized through single factor experiments, and the medium formula and culture conditions of the strain are initially obtained.

[0058] 3) Response surface analysis: According to the single factor results, further response surface analysis is used to determine the culture medium formula and culture conditions of the strain.

[0059] 4) Preparation of Bacillus subtilis zinc: Cultivate the domesticated Bacillus subtilis under the fin...

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Abstract

The invention relates to a zinc-rich bacterial strain and application thereof. The zinc-rich bacterial strain is characterized in that the bacillus subtilis is domesticated by gradually increasing theconcentration of zinc sulfate, so as to obtain a bacillus subtilis strain; the tolerance of the bacillus subtilis is obviously improved, the zinc content of the bacillus subtilis is increased, the content of crude protein, enzyme, small peptide, amino acid and the like in the bacillus subtilis strain is increased, the microecology is integrated with the organic zinc, the convenience in use is realized, and the application effect is good; the prepared bacillus subtilis strain zinc not only has the advantages of bacillus subtilis, but also the inorganic zinc is converted into thallus organic zinc, the utilization rate of trace metal elements by animals is greatly improved, the biology function of the organic zinc is well utilized, and the purpose of one bacterial strain with two functions is realized; because the utilization rate of organic zinc is improved, the usage amount and discharge amount of inorganic zinc in feed are greatly reduced, and the pollution to ecological environment is reduced; the important technical support is provided for the development of novel animal microecological preparations and 'amount-reduction' usage of trace elements.

Description

Technical field [0001] The invention belongs to the technical field of production of animal and poultry microecological preparations, and specifically relates to a zinc-rich strain and its application. Background technique [0002] Bacillus subtilis is a kind of beneficial bacteria in the intestinal tract, with strong resistance to stress, can secrete a variety of enzymes and vitamins, can enrich divalent metal ions, is not easily affected by various enzymes in the intestinal tract of livestock and poultry, and can enhance livestock and poultry immunity Strength and anti-stress ability, regulate the intestinal flora, improve growth performance and other functions. Mainly used in the field of animal feed and environmental protection. Studies have shown that adding Bacillus subtilis powder to animal feed can significantly increase animal feed intake and various growth indicators; on the other hand, use Bacillus subtilis to absorb divalent metal ions to deal with heavy metal enviro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P9/00C12R1/125
CPCC12N1/20C12P9/00C12N1/205C12R2001/125
Inventor 王宝维黄燕萍葛文华张名爱岳斌
Owner 山东德信生物科技有限公司
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