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Application of biological control agent for removing Candidatus Liberobacter asiaticum

A biological control agent and removal technology, applied in the direction of chemicals for biological control, microorganism-based methods, applications, etc., can solve the problems of inability to remove pathogens, resurgence, poisoning, etc.

Active Publication Date: 2018-06-29
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] First, use heavy pruning to restore the tree vigor of the diseased trees: carry out heavy pruning on the citrus trees, and keep the three major main branches and main side branches after short cutting. After two and a half years, strengthen the management of fertilizer and water and the management of diseases and insect pests , to restore the tree body so that it can bloom and bear fruit normally; but this method cannot remove the pathogen, and it will reappear in the later stage
[0010] Second, the pneumatic pressure injection system: the traditional injection method is still used in some places
However, the problem of root poisoning cannot be fundamentally solved, and it needs to be further improved with the treatment of the root system.
However, the problem of root poisoning cannot be fundamentally solved, and it needs to be further improved with the treatment of the root system.

Method used

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  • Application of biological control agent for removing Candidatus Liberobacter asiaticum
  • Application of biological control agent for removing Candidatus Liberobacter asiaticum
  • Application of biological control agent for removing Candidatus Liberobacter asiaticum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation and identification of Bacillus GJ1

[0034] 1. Separation of bacteria

[0035] This strain was isolated from citrus leaves for the first time in the laboratory. These leaves were disinfected with conventional 70% ethanol for 10 seconds, treated with 10% NaClO for 10 minutes and washed with sterile water for 5 times, and then added PBS at a ratio of 1:100 (pH 7) buffer solution, inoculate in LB medium, culture for 24-36h at 37℃, isolate multiple bacterial strains, conduct biological control test on HLB by culturing single strain and seedling root irrigation treatment, As a result, GJ1 was confirmed to be effective for HLB control.

[0036] 2. Identification

[0037] Based on the 16S-23S rDNA inter-region sequence (ITS), primers were designed, sequence amplification and sequencing were carried out. The measured sequence was as shown in SEQ ID No. 1. After sequencing and result comparison analysis, this study found that the spore The sequence of 23S rDNA frag...

Embodiment 2

[0040] Example 2 Application of Bacillus GJ1 in removing the pathogen of citrus young tree Huanglong disease:

[0041] The technological process of Bacillus GJ1 for treating young trees susceptible to citrus Huanglong disease is as follows figure 1 :

[0042] 1. Shake flask culture and inoculation of Bacillus GJ1: Prepare the medium according to the following medium components. After autoclaving, inoculate activated Bacillus GJ1 at a volume ratio of 1:10. Cultivate at 28°C for 48 hours. 600 When it reaches 0.8-1.3, the Bacillus GJ1 bacterial liquid is prepared, and the Bacillus GJ1 bacterial liquid is used for the root irrigation treatment of seedlings.

[0043] Table 1 Bacillus GJ1 medium composition

[0044] Numbering

Composition and content

1

Corn flour 1%

2

Soy flour 1%

3

(NH 4 ) 2 SO 4 0.5%

4

KH 2 PO 4 0.01%

5

The balance is water

pH

7.5

[0045] 2. Citrus Huanglongbing control treatment and detection method: use Bacillus GJ1 bacterial liquid to remove Huanglongb...

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Abstract

The invention discloses an application of biological control agent for removing Candidatus Liberobacter asiaticum. The biological control agent is prepared from bacillus GJ1, the bacillus GJ1 is conserved in the China Center for Type Culture Collection (CCTCC) in 28th July, 2017, and the conservation number is CCTCC M 2017456. The application comprises the following steps: removing the nursery stock in Liberobacter asiaticum infection by using the above bacillus GJ1 bacteria solution. The bacillus GJ1 fungicide has a certain detoxification effect for the Liberobacter asiaticum, and the aim ofremoving the pathogen can be achieved by repeatedly inoculating to the seventh treatment.

Description

Technical field [0001] The invention belongs to the technical field of biological control agents, and specifically relates to the application of a biological control agent in removing the pathogen of citrus young tree Huanglong disease. Background technique [0002] Bacillus sp. belongs to the genus Bacillus. During its growth, it can produce a series of metabolites that can inhibit the activity of fungi and bacteria, and have a strong inhibitory effect on plant pathogens. The bacteria is widely distributed in nature, easy to separate and cultivate, non-toxic and harmless to humans and animals, and does not pollute the environment; its metabolites are relatively rich, it has broad-spectrum antibacterial activity and strong resistance to stress, rapid growth and good stability. [0003] Bacillus is a kind of bacteria with broad-spectrum antibacterial activity. It has strong ability to produce secondary metabolites and can produce a variety of antibacterial substances. Research has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 姜玲赵秀云丁圆席姜乾坤孙亮南京
Owner HUAZHONG AGRI UNIV
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