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Live bacteria fresh-keeping agent and application thereof in fresh keeping of cultured fishes

The technology of a preservative and live bacteria is applied in the field of microbial preparations, which can solve the problems of high processing cost and high price, and achieve the effects of reducing the cost of preservation, prolonging the preservation period and changing the structure of the flora.

Active Publication Date: 2018-06-29
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Animal / plant-derived preservatives are limited by raw materials, and the price is high; while the current industrialized microbial-sourced preservatives are all microbial metabolites, the processing cost is high, and they are only used in high-value products

Method used

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  • Live bacteria fresh-keeping agent and application thereof in fresh keeping of cultured fishes
  • Live bacteria fresh-keeping agent and application thereof in fresh keeping of cultured fishes
  • Live bacteria fresh-keeping agent and application thereof in fresh keeping of cultured fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation of the living bacteria preservative containing Bacillus subtilis of the present invention specifically comprises the following steps:

[0023] (1) Seed liquid preparation: Take out the strains stored in the refrigerator at 4°C, pick a ring of bacteria and insert it into 100 mL beef extract peptone solid medium, culture at 37°C, 200r / min, for 1 day; beef extract peptone medium: 2.5g beef extract, 2.5g sodium chloride, 5g peptone, 500mL distilled water, pH7.0~7.2;

[0024] (2) Preparation of fermentation medium: yeast extract 14g, anhydrous glucose 14g, MgSO 4 1g, distilled water 1000mL, pH7.0;

[0025] (3) Preparation of fermentation broth of Bacillus subtilis bacteria: Inoculate the seed solution to the fermentation medium of step (2) at a ratio of 5%, and the liquid volume in the triangular flask is 100 mL, and cultured at 37°C and 150 r / min for 48 h, obtaining the fermentation broth;

[0026] (3) Obtain bacterial cells by centrifugation: centrifuge ...

Embodiment 2

[0029] (1) Feeding of tilapia. 130 juvenile tilapias of 20-30g were reared according to the following 4 groups of feeding methods. About 30-35 juvenile tilapias in each group were equally distributed and raised in two boxes of about 75L. The water temperature of each box is controlled at 28±1°C, and one-half of the water is changed every day. The feed was fed according to the proportion of 1% body weight per day, and after a one-week adaptation period with the normal feed, they were fed in groups according to the following treatment groups for 7 days.

[0030] Treatment group 1: in normal water body, fed with common feed. Feed the feed at a rate of 1% of body weight per day.

[0031] Treatment group 2: in normal water body, fed with probiotic feed. Feed Bacillus subtilis feed at a daily rate of 1% of body weight.

[0032] Treatment group 3: In the corrupt water body, add common feed feeding group. In corrupted water, add 10 on the 1st day of feeding 8 Individual / mL putr...

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Abstract

The invention provides a live bacteria fresh-keeping agent and an application thereof in the fresh keeping of cultured fishes. A bacillus subtilis live bacterial preparation is utilized to inhibit thegrowth of putrefying bacteria of fish bodies so as to achieve the purpose of prolonging refrigeration shelf life of the fishes. The bacillus subtilis is bacillus subtilis BSh0851. The fresh-keeping agent has the advantages that the bacillus subtilis fresh-keeping agent of the invention can inhibit the growth of the putrefying bacteria of the fish bodies, and alleviate the adhesion and breeding ofthe putrefying bacteria on fish intestines and fish gills at the source so as to achieve the purpose of prolonging fresh-keeping period.

Description

technical field [0001] The invention belongs to the field of microbial preparations, and in particular relates to a fresh-keeping agent for live bacteria and its application in fresh-keeping of cultured fish. Background technique [0002] Fresh-keeping technology is an important part of the aquatic product industry. It is the key to maintaining high prices, extending shelf life, expanding sales radius and prolonging processing time. It has important economic significance for the development of aquatic products. At present, the fresh-keeping technologies applied to aquatic products mainly include low-temperature preservation, chemical preservation, gas preservation, radiation preservation and biological preservation. Biological preservation has become a new hotspot for development due to its advantages of high safety and low energy consumption. Plant-derived preservatives mainly play an antioxidant role; animal-derived bacteriostatic agents such as protamine; microbial-deriv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K50/80A01K61/10
CPCA23K10/12A23K50/80A01K61/10Y02A40/81
Inventor 张雯张绍升倪莉王芳婷
Owner FUZHOU UNIV
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